CARBOHYDRATE-SPECIFIC ADHESION OF BACTERIA TO THIN-LAYER CHROMATOGRAMS - A RATIONALIZED APPROACH TO THE STUDY OF HOST-CELL GLYCOLIPID RECEPTORS
CARBOHYDRATE-SPECIFIC ADHESION OF BACTERIA TO THIN-LAYER CHROMATOGRAMS - A RATIONALIZED APPROACH TO THE STUDY OF HOST-CELL GLYCOLIPID RECEPTORS
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DOI：
10.1016/0003-2697(85)90410-5
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
THURIN, J
中科院分区：
文献类型：
--
作者：
HANSSON, GC;KARLSSON, KA;THURIN, J
Conditions were adapted for the binding of intact bacteria to glycosphingolipids in a thin-layer chromatogram. Bacteria [Propionibacterium granulosum] labeled externally with 125I or metabolically with other isotopes are layered on the plate and after repeated washing the bound bacteria are detected by autoradiography. Using this technique several kinds of bacteria adhere to the plate in a carbohydrate-specific way with practically no background binding. Among the advantages of the method is the possible detection of a minor receptor component of a complex mixture extracted from a target cell, facilitating the isolation of the receptor for structural studies. The multivalent solid-phase presentation of the receptor candidate should also reveal low-affinity binding sites, which may escape detection in traditional inhibition experiments with soluble oligosaccharides.
