Actin-myosin contractility is responsible for the reduced viability of dissociated human embryonic stem cells.
Actin-myosin contractility is responsible for the reduced viability of dissociated human embryonic stem cells.
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DOI：
10.1016/j.stem.2010.06.017
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发表时间：
2010-08-06
期刊：
影响因子：
23.9
通讯作者：
Thomson JA
中科院分区：
文献类型：
--
作者：
Chen G;Hou Z;Gulbranson DR;Thomson JA
人类胚胎干细胞是三种胚胎胚层的多能前体细胞。人类胚胎干细胞具有基底-根尖极性、连接复合物、整合素依赖的基质粘附和e -钙粘蛋白依赖的细胞-细胞粘附，这些都是完整哺乳动物胚胎外胚层上皮共有的特征。上皮结构破坏后，通常观察到程序性细胞死亡。如果个体化的人类胚胎干细胞被阻止重新附着和形成菌落，它们的生存能力就会大大降低。在这里，我们表明肌动蛋白-肌球蛋白收缩是人类胚胎干细胞解离后细胞死亡反应的关键效应因子。抑制肌凝蛋白重链atp酶，下调肌凝蛋白重链，下调肌凝蛋白轻链，均可提高人源性胚胎干细胞的存活率和克隆效率。ROCK抑制可降低肌球蛋白轻链磷酸化，提示抑制肌动蛋白-肌球蛋白收缩也是ROCK抑制剂提高人ES细胞克隆效率的机制。
Human ES cells are the pluripotent precursor of the three embryonic germ layers. Human ES cells exhibit basal-apical polarity, junctional complexes, integrin-dependent matrix adhesion, and E-cadherin-dependent cell-cell adhesion, all characteristics shared by the epiblast epithelium of the intact mammalian embryo. After disruption of epithelial structures, programmed cell death is commonly observed. If individualized human ES cells are prevented from reattaching and forming colonies, their viability is significantly reduced. Here we show that actin-myosin contraction is a critical effector of the cell death response to human ES cell dissociation. Inhibition of myosin heavy chain ATPase, downregulation of myosin heavy chain, and downregulation of myosin light chain all increase survival and cloning efficiency of individualized human ES cells. ROCK inhibition decreases phosphorylation of myosin light chain, suggesting that inhibition of actin-myosin contraction is also the mechanism through which ROCK inhibitors increase cloning efficiency of human ES cells.
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DOI：
10.1083/jcb.200802081
发表时间：
2008-06-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fackler OT;Grosse R
通讯作者：
Grosse R
影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
5.2
作者：
Krtolica, Ana;Genbacev, Olga;Fisher, Susan J.
通讯作者：
Fisher, Susan J.
影响因子：
4
作者：
Krawetz, Roman J.;Li, Xiangyun;Rancourt, Derrick E.
通讯作者：
Rancourt, Derrick E.
非肌肉肌球蛋白重链IIA在调节上皮顶连接处的独特作用。
影响因子：
3.7
作者：
Ivanov, Andrei I.;Bachar, Moshe;Babbin, Brian A.;Adelstein, Robert S.;Nusrat, Asma;Parkos, Charles A.
通讯作者：
Parkos, Charles A.