Immunization protocols against Cryptosporidium parvum in ovines:: protection in suckling lambs
Immunization protocols against Cryptosporidium parvum in ovines:: protection in suckling lambs
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DOI：
10.1016/j.vetpar.2004.11.033
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发表时间：
2005-04-20
影响因子：
2.6
通讯作者：
Rojo-Vázquez, F
中科院分区：
文献类型：
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作者：
Martín-Gómez, S;Alvarez-Sánchez, MA;Rojo-Vázquez, F
在36 - 48 h龄（感染后第0天）用10（6）个微小隐孢子虫卵囊实验感染的羔羊中，检测绵羊初乳和免疫母羊乳汁预防隐孢子虫病的能力。所有羔羊均感染并发展为临床隐孢子虫病。然而，免疫母鼠喂养的羔羊表现出脱落所涉及的，显着，更少的卵囊，并持续了较短的时间比对照组羔羊。此外，腹泻不太严重。用2ml弗氏完全佐剂和2ml C.在分娩前4周给予无菌磷酸盐缓冲盐水溶液中的parvum抗原，同时乳房内输注在2ml弗氏不完全佐剂中乳化的2ml无菌PBS中的25 μ g抗原，显示出最高的抗-C。乳汁分泌物中的微小滴度。在他们的情况下，卵囊输出的开始延迟了两天，专利期缩短了三天，他们的腹泻只持续了三天，卵囊脱落的数量减少了77%。结果是，在研究结束时，它们的活重比对照组的羔羊多增加了2公斤。这些结果表明，催乳免疫预防应有助于减轻小反刍动物隐孢子虫病造成的经济损失，以及通过减少环境中的卵囊污染来降低人类感染的风险。(c)2004 Elsevier B.V.保留所有权利。
Ovine colostrum and milk from immunized ewes were tested for their ability to prevent cryptosporidiosis in the lambs experimentally infected with 10(6) oocysts of Cryptosporidium parvum at 36-48 h of age (day 0 post-infection). All lambs became infected and developed clinical cryptosporidiosis. However, lambs fed by immunized dams have shown shedding involved, significantly, fewer oocysts and lasted for a shorter period than in control lambs. In addition, diarrhoea was less severe. The best results emerged in lambs of ewes immunized by intramuscular injection of an emulsion of 2 ml of Freund's complete adjuvant and 2 ml of C. parvum antigen in sterile phosphate buffered saline solution, administrated four weeks before parturition, together with an intramammary infusion of 25 mu g of antigen in 2 ml of sterile PBS emulsified in 2 ml of Freund's incomplete adjuvant, which showed the highest anti-C. parvum titres in lacteal secretions. In their case, the onset of output of oocysts was delayed by two days, the patent period was shortened by three days, their diarrhoea continued for only three days, and the quantity of oocysts shed decreased by 77%. The outcome was that at the end of the study they had a live weight gain of 2 kg more than the lambs in the control group. These results indicate that lactogenic immunoprophylaxis should help mitigate the financial losses caused by cryptosporidiosis in small ruminants, as well as reducing the risk of infection of humans through the decreased contamination of the environment with oocysts. (c) 2004 Elsevier B.V. All rights reserved.