Plant presence and species combination, but not diversity, influence denitrifier activity and the composition of nirK-type denitrifier communities in grassland soil
Plant presence and species combination, but not diversity, influence denitrifier activity and the composition of nirK-type denitrifier communities in grassland soil
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DOI：
10.1111/j.1574-6941.2009.00732.x
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发表时间：
2009-12-01
影响因子：
4.2
通讯作者：
Conrad, Ralf
中科院分区：
文献类型：
--
作者：
Bremer, Christina;Braker, Gesche;Conrad, Ralf
为了探索植物群落、土壤反硝化菌和反硝化菌功能之间的潜在联系，连续两年研究了人工草地植物组合的存在、多样性（即物种丰富度）和植物组合对 nirK 型反硝化菌群落组成和反硝化菌活性的影响。建立了包含零个、四个和八个物种以及两个物种的不同组合的中生态系统。对编码含铜亚硝酸还原酶的 PCR 扩增 nirK 基因片段进行末端限制性片段长度多态性分析后，通过典型对应分析来分析反硝化菌群落组成的差异。作为反硝化菌功能的衡量标准，测定了土壤样品中的反硝化酶活性（DEA）。植物的存在以及组合和采样时间影响 nirK 型反硝化菌群落和 DEA 的组成，但不影响植物多样性。在植物存在的情况下，反硝化菌活性显着增加，尤其是在夏季和秋季生长时。总体而言，我们发现反硝化菌群落组成和功能之间存在强大而直接的联系，而且植物对反硝化菌功能具有额外的影响，这不能仅仅通过它们对 nirK 型反硝化菌群落组成的影响来解释。
To explore potential links between plant communities, soil denitrifiers and denitrifier function, the impact of presence, diversity (i.e. species richness) and plant combination on nirK-type denitrifier community composition and on denitrifier activity was studied in artificial grassland plant assemblages over two consecutive years. Mesocosms containing zero, four and eight species and different combinations of two species were set up. Differences in denitrifier community composition were analysed by canonical correspondence analyses following terminal restriction fragment length polymorphism analysis of PCR-amplified nirK gene fragments coding for the copper-containing nitrite reductase. As a measure of denitrifier function, denitrifier enzyme activity (DEA) was determined in the soil samples. The presence as well as the combination of plants and sampling time, but not plant diversity, affected the composition of the nirK-type denitrifier community and DEA. Denitrifier activity significantly increased in the presence of plants, especially when they were growing during summer and autumn. Overall, we found a strong and direct linkage of denitrifier community composition and functioning, but also that plants had additional effects on denitrifier function that could not be solely explained by their effects on nirK-type denitrifier community composition.