Mechanistic insight into the elastin degradation process by the metalloprotease myroilysin from the deep-sea bacterium Myroides profundi D25.
Mechanistic insight into the elastin degradation process by the metalloprotease myroilysin from the deep-sea bacterium Myroides profundi D25.
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深海 Myroides profundi D25 金属蛋白酶 Myroilysin 降解弹性蛋白过程的机制研究
DOI：
10.3390/md13031481
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发表时间：
2015-03-18
期刊：
影响因子：
5.4
通讯作者：
Zhang YZ
中科院分区：
文献类型：
--
作者：
Yang J;Zhao HL;Tang BL;Chen XL;Su HN;Zhang XY;Song XY;Zhou BC;Xie BB;Weiss AS;Zhang YZ
Elastases have been widely studied because of their important uses as medicine and meat tenderizers. However, there are relatively few studies on marine elastases. Myroilysin, secreted by Myroides profundi D25 from deep-sea sediment, is a novel elastase. In this study, we examined the elastin degradation mechanism of myroilysin. When mixed with insoluble bovine elastin, myroilysin bound hydrophobically, suggesting that this elastase may interact with the hydrophobic domains of elastin. Consistent with this, analysis of the cleavage pattern of myroilysin on bovine elastin and recombinant tropoelastin revealed that myroilysin preferentially cleaves peptide bonds with hydrophobic residues at the P1 and/or P1′ positions. Scanning electron microscopy (SEM) of cross-linked recombinant tropoelastin degraded by myroilysin showed preferential damages of spherules over cross-links, as expected for a hydrophobic preference. The degradation process of myroilysin on bovine elastin fibres was followed by light microscopy and SEM, revealing that degradation begins with the formation of crevices and cavities at the fibre surface, with these openings increasing in number and size until the fibre breaks into small pieces, which are subsequently fragmented. Our results are helpful for developing biotechnological applications for myroilysin.
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DOI：
10.1161/atvbaha.110.219147
发表时间：
2011-03
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Johnson JL;Devel L;Czarny B;George SJ;Jackson CL;Rogakos V;Beau F;Yiotakis A;Newby AC;Dive V
通讯作者：
Dive V
影响因子：
5.4
作者：
Heinz, Andrea;Jung, Michael C.;Schmelzer, Christian E. H.
通讯作者：
Schmelzer, Christian E. H.
影响因子：
15.6
作者：
Yeo, Giselle C.;Keeley, Fred W.;Weiss, Anthony S.
通讯作者：
Weiss, Anthony S.
DOI：
10.1073/pnas.1014280108
发表时间：
2011-03-15
影响因子：
11.1
作者：
Baldock, Clair;Oberhauser, Andres F.;Weiss, Anthony S.
通讯作者：
Weiss, Anthony S.
影响因子：
4.8
作者：
Wu, WJ;Vrhovski, B;Weiss, AS
通讯作者：
Weiss, AS