The molecular network governing nodule organogenesis and infection in the model legume Lotus japonicus.
The molecular network governing nodule organogenesis and infection in the model legume Lotus japonicus.
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DOI：
10.1038/ncomms1009
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发表时间：
2010-04-12
影响因子：
16.6
通讯作者：
Stougaard, Jens
中科院分区：
文献类型：
--
作者：
Madsen, Lene H.;Tirichine, Leila;Jurkiewicz, Anna;Sullivan, John T.;Heckmann, Anne B.;Bek, Anita S.;Ronson, Clive W.;James, Euan K.;Stougaard, Jens
豆科植物根瘤内部组织的细菌感染是在表皮细胞层控制的，并且与正在进行的器官发育密切协调。利用自发结瘤的百脉根植物突变体将根瘤器官发生与感染分离，我们确定了 16 个基因在这两个发育过程中的作用。我们表明，宿主编码机制控制着表皮、根皮层和单细胞水平上的三种替代进入过程。单细胞感染不涉及跨细胞感染线的形成，并且独立于宿主Nod因子受体和细菌Nod因子信号。相比之下，表皮根毛感染线需要Nod因子感知，而次级Ca2+振荡之前的初级信号转导基因则具有间接作用。我们通过在大多数现存豆科植物中观察到的直接细胞间表皮入侵以及随后的裂缝进入和根毛入侵的进化来为根瘤菌感染的起源提供支持。一些物种中的植物和细菌共生导致固氮结核的协同形成和植物宿主的感染。 Madsen 及其同事利用突变的百脉根植物确定了 16 个不同基因在这两个过程中的作用。
Bacterial infection of interior tissues of legume root nodules is controlled at the epidermal cell layer and is closely coordinated with progressing organ development. Using spontaneous nodulating Lotus japonicus plant mutants to uncouple nodule organogenesis from infection, we have determined the role of 16 genes in these two developmental processes. We show that host-encoded mechanisms control three alternative entry processes operating in the epidermis, the root cortex and at the single cell level. Single cell infection did not involve the formation of trans-cellular infection threads and was independent of host Nod-factor receptors and bacterial Nod-factor signals. In contrast, Nod-factor perception was required for epidermal root hair infection threads, whereas primary signal transduction genes preceding the secondary Ca2+ oscillations have an indirect role. We provide support for the origin of rhizobial infection through direct intercellular epidermal invasion and subsequent evolution of crack entry and root hair invasions observed in most extant legumes. Plant and bacteria symbiosis in some species results in the coordinate formation of nitrogen fixing nodules and infection of the plant host. Using mutant Lotus japonicus plants, Madsen and colleagues have determined the role of 16 different genes in these two processes.
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DOI：
10.1073/pnas.0504250102
发表时间：
2005-07-19
影响因子：
11.1
作者：
Capoen, W;Goormachtig, S;Holsters, M
通讯作者：
Holsters, M
影响因子：
3.5
作者：
D'Haeze, W;Gao, MS;Holsters, M
通讯作者：
Holsters, M
影响因子：
7.4
作者：
Arrighi, Jean-Francois;Barre, Annick;Gough, Clare
通讯作者：
Gough, Clare
影响因子：
4.2
作者：
James, EK;Minchin, FR;Sprent, JI
通讯作者：
Sprent, JI
DOI：
10.1073/pnas.0504284102
发表时间：
2005-07-19
影响因子：
11.1
作者：
Limpens, E;Mirabella, R;Geurts, R
通讯作者：
Geurts, R