Mechanically induced calcium signaling in chondrocytes in situ
Mechanically induced calcium signaling in chondrocytes in situ
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DOI：
10.1002/jor.21536
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发表时间：
2012-03-01
影响因子：
2.8
通讯作者：
Herzog, Walter
中科院分区：
文献类型：
--
作者：
Han, Sang-Kuy;Wouters, Wim;Herzog, Walter
细胞内钙（Ca2+）浓度的变化，也被称为Ca2+信号，已被广泛研究在关节软骨软骨细胞中，以研究机械转导的途径。各种物理刺激可以产生Ca2+流入细胞，这反过来被认为会触发一系列代谢和信号传导过程。与大多数研究相反，本研究中使用的方法允许连续真实的时间记录软骨细胞在其天然环境中的钙信号。因此，细胞与细胞外基质（ECM）的相互作用得到充分考虑。对动态负载条件和不同温度下的钙信号传导进行定量。钙信号的峰值幅度在37 ℃下比在21 ℃下更大且持续时间更短。此外，Ca2+信号参与了更大比例的细胞在动态相比，放松阶段的负荷。与在琼脂糖凝胶中接种的分离的软骨细胞中观察到的时间延迟的信号传导相反，原位的Ca2+信号传导几乎是瞬时的，以响应动态负荷。原位和体外细胞信号传导反应之间的这些差异可能提供关键的洞察ECM的作用，在完整的软骨中提供机械转导的途径，是不存在的分离的细胞接种在凝胶结构。(C)2011年由Wiley Periodicals，Inc.出版的骨科研究学会。J Orthop Res 30：475481，2012
Changes in intracellular calcium (Ca2+) concentration, also known as Ca2+ signaling, have been widely studied in articular cartilage chondrocytes to investigate pathways of mechanotransduction. Various physical stimuli can generate an influx of Ca2+ into the cell, which in turn is thought to trigger a range of metabolic and signaling processes. In contrast to most studies, the approach used in this study allows for continuous real time recording of calcium signals in chondrocytes in their native environment. Therefore, interactions of cells with the extracellular matrix (ECM) are fully accounted for. Calcium signaling was quantified for dynamic loading conditions and at different temperatures. Peak magnitudes of calcium signals were greater and of shorter duration at 37 degrees C than at 21 degrees C. Furthermore, Ca2+ signals were involved in a greater percentage of cells in the dynamic compared to the relaxation phases of loading. In contrast to the time-delayed signaling observed in isolated chondrocytes seeded in agarose gel, Ca2+ signaling in situ is virtually instantaneous in response to dynamic loading. These differences between in situ and in vitro cell signaling responses might provide crucial insight into the role of the ECM in providing pathways of mechanotransduction in the intact cartilage that are absent in isolated cells seeded in gel constructs. (C) 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:475481, 2012