Preclinical applications of imaging for cancer gene therapy
Preclinical applications of imaging for cancer gene therapy
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DOI：
10.1017/s1462399406000044
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发表时间：
2006-07-14
影响因子：
6.2
通讯作者：
Vassaux, Georges
中科院分区：
文献类型：
--
作者：
Briat, Arnaud;Vassaux, Georges
在实验性癌症治疗中，基因治疗是一个非常有吸引力的策略。理想情况下，这种方法的目标是有选择地将治疗性基因输送到癌细胞。然而，由于难以测量转基因传递和在体内量化转基因表达，基因治疗配方的改进进展受到了阻碍。在临床试验中，终点几乎完全依赖于通过分子和组织病理学方法进行的活检分析，这些方法提供的信息有限。因此，为了确保基因治疗的合理发展，一个关键的问题是利用基因递送载体对体内基因表达进行非侵入性监测的技术。这样的成像技术将允许产生关于基因表达的定量信息，并评估癌症基因治疗的效果。在过去的十年里，活体分子成像领域取得了进展。这篇综述着重介绍了目前在临床前模型中开发的各种方法。
Gene therapy is a very attractive strategy in experimental cancer therapy. Ideally, the approach aims to deliver therapeutic genes selectively to cancer cells. However, progress in the improvement of gene therapy formulations has been hampered by difficulties in measuring transgene delivery and in quantifying transgene expression in vivo. In clinical trials, endpoints rely almost exclusively on the analysis of biopsies by molecular and histopathological methods, which provide limited information. Therefore, to ensure the rational development of gene therapy, a crucial issue is the utilisation of technologies for the non-invasive monitoring of spatial and temporal gene expression in vivo upon administration of a gene delivery vector. Such imaging technologies would allow the generation of quantitative information about gene expression and the assessment of cancer gene therapy efficacy. In the past decade, progress has been made in the field of in vivo molecular imaging. This review highlights the various methods currently being developed in preclinical models.