Nondestructive FTIR monitoring of leaf senescence and elicitin-induced changes in plant leaves
Nondestructive FTIR monitoring of leaf senescence and elicitin-induced changes in plant leaves
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DOI：
10.1002/bip.10297
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发表时间：
2003-01-01
期刊：
影响因子：
2.9
通讯作者：
Singh, BR
中科院分区：
文献类型：
--
作者：
Ivanova, DG;Singh, BR
研究了FTIR衰减全反射技术在植物叶片衰老等生理过程原位监测中的应用。从老叶光谱中减去幼叶光谱得到的差异光谱在1650-1500 cm（-1）处显示阳性条带，表明黑樱桃和甜椒灌木老叶中酚类物质的相对浓度较高。烟草叶片的长期生理胁迫表现出在3475 cm（-1）附近的吸光度随时间逐渐增加，对应于羟基官能团。在1650和1500 cm（-1）之间也观察到吸收变化，这可能对应于酚类化合物。生理和诱导老化导致的FTIR吸收光谱的特征变化也被检测为对用重组α-激发素，肉桂素处理的响应。这允许使用光谱方法首次定量重组激发素的生物活性。我们建议，FTIR光谱提供了重要的信息发生在植物组织中的生理事件在体内，它可能是有用的植物对真菌毒素，如elicitins反应的原位表征。(C)2003 Wiley Periodicals，Inc.
The applicability of the FTIR attenuated total reflectance technique for in situ monitoring of plant physiological processes such as leaf senescence and aging has been examined. Difference spectra obtained by subtracting the spectrum of the young plant leaf from that of the older one revealed positive bands at 1650-1500 cm(-1), indicating a higher relative concentration of phenolics in the older leaves of both black cherry and sweet pepper bush leaves. Prolonged physiological stress of tobacco leaves exhibited a progressive time-dependent increase of the absorbance at around 3475 cm(-1), corresponding to hydroxyl functional groups. Absorption changes were also observed between 1650 and 1500 cm(-1), which are likely to correspond to phenolics. The characteristic changes of the FTIR absorbance spectra resulting from physiological and induced aging were detected also as a response to treatment with a recombinant a-elicitin, cinnamomin. This allowed the first quantification of the biological activity of a recombinant elicitin using a spectroscopic method. We suggest that FTIR spectroscopy provides important information about physiological events occurring in plant tissue in vivo, and it could be useful for the in situ characterization of the plant responsiveness to fungal toxins such as elicitins. (C) 2003 Wiley Periodicals, Inc.