HLA class II polymorphisms associated with the physiologic characteristics defined by traditional Chinese medicine: Linking modern genetics with an ancient medicine
HLA class II polymorphisms associated with the physiologic characteristics defined by traditional Chinese medicine: Linking modern genetics with an ancient medicine
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DOI：
10.1089/acm.2006.6126
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发表时间：
2007-03-01
影响因子：
2.6
通讯作者：
Xu, Anlong
中科院分区：
文献类型：
--
作者：
Chen, Shangwu;Lv, Fengjuan;Xu, Anlong
目的：探讨人类白细胞抗原(HLA1)基因多态性与中医体质分类的关系。设计：以《中医体质学理论》为标准，将南中国汉族700人分为7个体质组，采用聚合酶链式反应-测序法对DRB1、DPB1、DQB1基因进行分型。结果：DPB1*0501在阴虚组，DRB1*09012在痰湿组，DRB1*0501在阴虚组，DRB1*09012在痰湿组，DRB1*0501在阴虚组，DRB1*0501在痰湿组，气虚、痰湿两组的DQB1*03032等位基因与正常体质对应的等位基因有显著差异，提示这些等位基因可能是群体特有的等位基因，与特定体质有关。结论：本研究首次采用高分辨分型技术系统地探讨了人类白细胞抗原(HL A)与中医体质的关系。这些结果为中医体质分类提供了遗传学依据。这项研究首次为使用现代生物学方法深入了解传统医学理论奠定了基础。
Objectives: The aim of this study was to test whether human leukocyte antigen (HLA) polymorphism contributes to the physical constitutions classified in Traditional Chinese Medicine (TCM).Design: Seven hundred six (706) individuals of the Han ethnic group inhabiting South China were classified into 7 TCM constitution groups, according to the criteria described in Theories of Physical Constitutions of Traditional Chinese Medicine, and the distributions of HLA-DRB1, DPB1, and DQB1 were investigated using the polymerase chain reaction-sequencing-based typing method.Results: The allele frequencies of DPB1*0501 in the Yin-deficiency group, DRB1*09012 in the Phlegm-wetness group, and DQB1*03032 in the Qi-deficiency and Phlegm-wetness groups were significantly different from that of the corresponding alleles in the Normality constitution, suggesting those alleles might be group-specific alleles and thus related to a particular constitution. Based on our analysis of serological groups of HLA, the associations of DR*04 with the Blood-stasis group and DQ*09 with the Qi-deficiency and Phlegm-wetness groups were observed.Conclusions: This was the first study to systematically investigate the relationship between HLA and TCM constitution using a high-resolution typing technique. The results suggested a genetic basis for the classification of physical constitution in TCM. This study laid the foundation, for the first time ever, toward gaining insight into the theory of traditional medicine using modern biological approaches.