CLP36 interacts with palladin in dorsal root ganglion neurons
CLP36 interacts with palladin in dorsal root ganglion neurons
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DOI：
10.1016/j.neulet.2010.03.081
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发表时间：
2010-05-31
影响因子：
2.5
通讯作者：
Sato, Kohji
中科院分区：
文献类型：
--
作者：
Hasegawa, Tomohiko;Ohno, Koji;Sato, Kohji
CLP 36是α-辅肌动蛋白相关的LIM蛋白（ALP）/enigma蛋白家族的成员，在周围神经系统中的神经突生长中起作用。然而，其潜在的分子机制尚不清楚。在这项研究中，我们进行了酵母双杂交筛选的E18小鼠全身cDNA文库与CLP 36作为诱饵和分离palladin作为CLP 36结合蛋白。Palladin是一种肌动蛋白结合蛋白，它被证明在皮层神经元的延伸中具有作用。免疫共沉淀研究表明，CLP 36和palladin在背根神经节（DRG）中形成复合物。此外，CLP 36和palladin共定位于原代DRG神经元的神经突和细胞体中。此外，坐骨神经切断增加了DRG神经元中CLP 36和palladin mRNA的表达，其中CLP 36 mRNA的增加更为突出。这意味着CLP 36在神经再生中具有比palladin更特异的作用。我们的研究结果表明，CLP 36可能与palladin相互作用，影响坐骨神经再生过程中的轴突生长。(C)2010爱思唯尔爱尔兰有限公司版权所有。
CLP36, a member of the a-actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. However, the underlying molecular mechanisms are not known. In this study, we performed yeast two-hybrid screening of an E18 mouse whole-body cDNA library with CLP36 as the bait and isolated palladin as a CLP36-binding protein. Palladin is an actin-binding protein and it was shown to have a role in the extension of cortical neurons. A coimmunoprecipitation study showed that CLP36 and palladin formed a complex in the dorsal root ganglion (DRG). In addition, CLP36 and palladin were colocalized in the neurites and cell bodies of primary DRG neurons. Furthermore, sciatic nerve transection increased the expression of both CLP36 and palladin mRNAs in DRG neurons, with the increase in CLP36 mRNA being more prominent. This implies that CLP36 has a more specific role in nerve regeneration than palladin. Our results suggest that CLP36 may interact with palladin to influence neurite outgrowth during sciatic nerve regeneration. (C) 2010 Elsevier Ireland Ltd. All rights reserved.