INTRACELLULAR OXIDATION- REDUCTION STATES IN VIVO
INTRACELLULAR OXIDATION- REDUCTION STATES IN VIVO
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DOI：
10.1126/science.137.3529.499
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发表时间：
1962-01-01
期刊：
影响因子：
56.9
通讯作者：
COHEN, P
中科院分区：
文献类型：
--
作者：
CHANCE, B;JOBSIS, F;COHEN, P
现在似乎有可能连续记录细胞内氧化还原水平的变化，在各种组织和器官的线粒体中还原的吡啶核苷酸的荧光原位。对大鼠肾脏和大脑皮层的研究表明，荧光的变化不会受到氧合血红蛋白的影响。N、S、Cn和Co引起荧光增加到非常接近相同的水平，并且这种增加归因于线粒体二磷酸吡啶核苷酸的较大减少。阿米妥在低血药浓度下引起肾皮质减少，在高血药浓度下引起脑皮质减少。吡啶核苷酸对低氧浓度的定性反应表明大脑比肾脏更敏感。在8%的吸入氧浓度下，在大脑上观察到的吡啶核苷酸减少的第一个可测量的增加发生。当大脑中吡啶核苷酸减少的百分比增加达到约90时，呼吸停止;此时肾脏中的百分比增加仅为约30。这种差异大致对应于O2张力的十倍差异。大脑中吡啶核苷酸还原的半数最大增加发生在吸入氧浓度约为4%时，对应于约0.2 mm的细胞内O2张力（47）。
It now appears to be possible to continuously record changes in intracellular oxidation-reduction levels in terms of the fluorescence of reduced pyridine nucleotide in mitochondria of various tissues and organs in situ. Studies of kidney and brain cortex in the rat show that changes in fluorescence are not measurably affected by the presence of oxyhemoglobin. N, S, Cn, and Co cause increases in fluorescence to very nearly the same levels, and the increases are attributed to larger reduction of mito-chondrial diphosphopyridine nucleotide. Amytal at a low blood concentration causes increased reduction in the kidney cortex, and at a high blood concentration, in the brain cortex. The qualitative response of the pyridine nucleotide to low oxygen concentrations shows the brain to be more sensitive than the kidney. The 1st measurable increase in pyridine nucleotide reduction observed on the brain occurs at a concentration of inspired oxygen of 8%. Breathing stops when the percentage increase of pyridine nucleotide reduction on the brain reaches about 90; at this point the percentage increase for the kidney is only about 30. This difference corresponds roughly to a tenfold difference in O2 tension. Half-maximal increase in pyridine nucleotide reduction on the brain occurs at a concentration of inspired oxygen of about 4% and corresponds to an intracellular O2 tension of about 0.2 mm (47).