Bioaerosol emissions and detection of airborne antibiotic resistance genes from a wastewater treatment plant
Bioaerosol emissions and detection of airborne antibiotic resistance genes from a wastewater treatment plant
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废水处理厂的生物气溶胶排放和空气中抗生素抗性基因的检测
DOI：
10.1016/j.atmosenv.2015.06.030
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发表时间：
2016-01-01
影响因子：
5
通讯作者：
Yao, Maosheng
中科院分区：
文献类型：
--
作者：
Li, Jing;Zhou, Liantong;Yao, Maosheng
使用路透高流量离心采样器 (RCS) 从北京某污水处理厂 (WWTP) 的 12 个采样点（包括 7 个厂内采样点、1 个上风向采样点和 4 个下风向采样点）采集空气样本；并使用培养和高通量基因序列研究了它们的微生物组分。此外，还使用紫外线空气动力学粒径分析仪 (UV-APS) 对 7 个植物内位点的活（荧光）生物气溶胶浓度进行了监测，每个位点 30 分钟。采用聚合酶链式反应（PCR）结合凝胶电泳技术，对从工厂采集的空气和水样中可能存在的细菌抗生素抗性基因和整合子进行了研究。结果显示，污泥浓缩池附近的空气检测到最高水平的可培养细菌气溶胶（高达1697 CFU/m(3)）和真菌气溶胶（高达930 CFU/m(3)）。 CFU/m(3))。除办公楼在1.5μm处有荧光峰外，大多数采样点均在3～4μm左右观察到荧光峰，数量浓度水平高达1233～6533个/m(3)。从生物反应池收集的空气样本中检测到约 300 种独特的细菌，包括人类机会性病原体，如睾丸酮丛毛单胞菌和奥斯陆莫拉氏菌。此外，我们还从筛选室和生物反应池收集的空气样本中检测到了对复方新诺明（又称septra、bactrim和TMP-SMX）耐药的sul2基因和1类整合酶基因。总体而言，筛选室、污泥浓缩池和生物反应池带来了显着的微生物暴露风险，包括来自空气中抗生素抗性基因的风险。 (C) 2015 Elsevier Ltd. 保留所有权利。
Air samples from twelve sampling sites (including seven intra-plant sites, one upwind site and four downwind sites) from a wastewater treatment plant (WWTP) in Beijing were collected using a Reuter Centrifugal Sampler High Flow (RCS); and their microbial fractions were studied using culturing and high throughput gene sequence. In addition, the viable (fluorescent) bioaerosol concentrations for 7 intra-plant sites were also monitored for 30 min each using an ultraviolet aerodynamic particle sizer (UV-APS). Both air and water samples collected from the plant were investigated for possible bacterial antibiotic resistance genes and integrons using polymerase chain reaction (PCR) coupled with gel electrophoresis.The results showed that the air near sludge thickening basin was detected to have the highest level of culturable bacterial aerosols (up to 1697 CFU/m(3)) and fungal aerosols (up to 930 CFU/m(3)). For most sampling sites, fluorescent peaks were observed at around 3-4 mu m, except the office building with a peak at 1.5 mu m, with a number concentration level up to 1233-6533 Particles/m(3). About 300 unique bacterial species, including human opportunistic pathogens, such as Comamonas Testosteroni and Moraxella Osloensis, were detected from the air samples collected over the biological reaction basin. In addition, we have detected the sul2 gene resistant to cotrimoxazole (also known as septra, bactrim and TMP-SMX) and class 1 integrase gene from the air samples collected from the screen room and the biological reaction basin. Overall, the screen room, sludge thickening basin and biological reaction basin imposed significant microbial exposure risks, including those from airborne antibiotic resistance genes. (C) 2015 Elsevier Ltd. All rights reserved.