Production of new extracellular glycolipids by a strain of Cellulomonas cellulans (Oerskovia xanthineolytica) and their structural characterization
Production of new extracellular glycolipids by a strain of Cellulomonas cellulans (Oerskovia xanthineolytica) and their structural characterization
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DOI：
10.1139/cjm-44-3-238
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发表时间：
1998-03-01
影响因子：
2.8
通讯作者：
Vandecasteele, JP
中科院分区：
文献类型：
--
作者：
Arino, S;Marchal, R;Vandecasteele, JP
从土壤样品中分离出糖脂产生菌。其中一种菌株被鉴定为纤维素单胞菌（Oerskovia xanthineolytica），发现其产生大量不寻常的细胞外糖脂，这些糖脂至少由11种化合物组成。糖脂混合物的水解和气相色谱-质谱分析揭示了C-10至C-18范围内的脂肪酸和羟基脂肪酸的存在，其中16种被鉴定。缩水甘油部分由葡萄糖、鼠李糖和核糖组成。发现纤维单胞菌的细胞壁中存在相同的糖，其也含有极性脂质，包括糖脂。在菌株培养过程中，当培养基中的氮耗尽时，糖脂分泌受到刺激。在这些条件下，以葡萄糖当量表示的甘油发酵罐中的产量达到8.9 g/L。细胞疏水性在生长阶段上升到95%，在生产阶段下降到50%。总体结果表明，细菌细胞壁参与了这些新的细胞外糖脂的合成。
Glycolipid-producing bacteria were isolated from soil samples. One of the strains, identified as Cellulomonas cellulans (Oerskovia xanthineolytica), was found to produce significant amounts of unusual extracellular glycolipids, which were shown to be composed of at least 11 individual compounds. Hydrolysis of the glycolipid mixture and gas chromatography - mass spectrometry analysis revealed the presence of fatty acids and hydroxy fatty acids ranging from C-10 to C-18, 16 of which were identified. The glycidic moiety consisted of glucose, rhamnose, and ribose. The same sugars were found to be present in the cell wall of Cellulomonas cellulans, which also contained polar lipids including glycolipids. During strain cultivation, glycolipid excretion was stimulated when nitrogen was exhausted from the culture medium. In these conditions, the production in fermenters on glycerol, expressed in glucose equivalents, reached 8.9 g/L. Cell hydrophobicity, which rose to 95% during the growth phase, decreased to 50% during the production phase. The overall results show that the bacterial cell wall is involved in the synthesis of these new extracellular glycolipids.