Introgression of "QTL-hotspot" region enhances drought tolerance and grain yield in three elite chickpea cultivars
Introgression of "QTL-hotspot" region enhances drought tolerance and grain yield in three elite chickpea cultivars
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DOI：
10.1002/tpg2.20076
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发表时间：
2021-01-22
期刊：
影响因子：
4.2
通讯作者：
Varshney, Rajeev K.
中科院分区：
文献类型：
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作者：
Bharadwaj, Chellapilla;Tripathi, Shailesh;Varshney, Rajeev K.
为了利用分子标记辅助回交（MABC）方法提高大豆的耐旱性，我们将ICC 4958中含有几个耐旱相关性状的数量性状基因座（QTL）的“QTL热点”区域基因渗入到3个优良的印度鹰嘴豆（Cicer arietinum L.）栽培品种：Pusa 372、Pusa 362和DCP 92-3。在QTL热点区域的8个SSR标记中，2 ~ 3个多态标记用于各自组合的前景选择。在分别在Pusa 372、Pusa 362和DCP 92-3的遗传背景中开发的渐渗系（IL）的情况下，总共47、53和46个SSR用于背景选择。总共开发了61个IL（Pusa 372中有20个BC 3 F3; Pusa 362中有20个BC 2 F3，DCP 92-3中有21个BC 3 F3），重现性亲本基因组恢复率>90%。不同遗传背景的6个改良系（例如Pusa 372中的BGM 10216; Pusa 362中的BG 3097和BG 4005; DCP 92-3中的IPC（L4-14）、IPC（L4-16）和IPC（L19-1））显示出比它们各自的轮回亲本更好的性能。BGM 10216比Pusa 372增产16%，已被印度政府农业和农民福利部农作物标准、农作物品种通知和发布中央小组委员会发布为Pusa鹰嘴豆10216，用于印度的商业种植。总之，本研究报告的QTL-热点的渐渗，提高产量在干旱条件下，几个渐渗系的发展，并通过在印度的分子育种开发的Pusa鹰嘴豆10216的释放。
With an aim of enhancing drought tolerance using a marker-assisted backcrossing (MABC) approach, we introgressed the "QTL-hotspot" region from ICC 4958 accession that harbors quantitative trait loci (QTLs) for several drought-tolerance related traits into three elite Indian chickpea (Cicer arietinum L.) cultivars: Pusa 372, Pusa 362, and DCP 92-3. Of eight simple sequence repeat (SSR) markers in the QTL-hotspot region, two to three polymorphic markers were used for foreground selection with respective cross-combinations. A total of 47, 53, and 46 SSRs were used for background selection in case of introgression lines (ILs) developed in genetic backgrounds of Pusa 372, Pusa 362, and DCP 92-3, respectively. In total, 61 ILs (20 BC3F3 in Pusa 372; 20 BC2F3 in Pusa 362, and 21 BC3F3 in DCP 92-3), with >90% recurrent parent genome recovery were developed. Six improved lines in different genetic backgrounds (e.g. BGM 10216 in Pusa 372; BG 3097 and BG 4005 in Pusa 362; IPC(L4-14), IPC(L4-16), and IPC(L19-1) in DCP 92-3) showed better performance than their respective recurrent parents. BGM 10216, with 16% yield gain over Pusa 372, has been released as Pusa Chickpea 10216 by the Central Sub-Committees on Crop Standards, Notification and Release of Varieties of Agricultural Crops, Ministry of Agriculture and Farmers Welfare, Government of India, for commercial cultivation in India. In summary, this study reports introgression of the QTL-hotspot for enhancing yield under rainfed conditions, development of several introgression lines, and release of Pusa Chickpea 10216 developed through molecular breeding in India.