ENZYME LOCALIZATION IN THE ANAEROBIC MITOCHONDRIA OF ASCARIS LUMBRICOIDES
ENZYME LOCALIZATION IN THE ANAEROBIC MITOCHONDRIA OF ASCARIS LUMBRICOIDES
复制标题
蛔虫厌氧线粒体中酶的定位
作者：
R. Rew;H. J. Saz
本文报道了人蛔虫肌内线粒体的形态学观察。在生理条件下，suum在电子传递相关磷酸化中厌氧地起作用。这些蠕虫细胞器已被分馏成内外膜，基质和膜间空间馏分。酶系统的分布进行了测定，并与哺乳动物线粒体中相应的分布报告。琥珀酸和丙酮酸脱氢酶以及NADH氧化酶，Mg++依赖的ATP酶，腺苷酸激酶，柠檬酸合成酶，细胞色素c还原酶被确定为分布在哺乳动物线粒体。与哺乳动物系统相反，主要从蠕虫线粒体的膜间隙部分分离出腺苷酸酶和NAD连接的“苹果酸”酶。这些酶是蛔虫体内厌氧能量产生系统所必需的，并有望在膜间隙产生NADH。需要和可能的机制的质子易位系统，以获得能量产生的建议。
Mitochondria from the muscle of the parasitic nematode Ascaris lumbricoides var. suum function anaerobically in electron transport-associated phosphorylations under physiological conditions. These helminth organelles have been fractionated into inner and outer membrane, matrix, and intermembrane space fractions. The distributions of enzyme systems were determined and compared with corresponding distributions reported in mammalian mitochondria. Succinate and pyruvate dehydrogenases as well as NADH oxidase, Mg++-dependent ATPase, adenylate kinase, citrate synthase, and cytochrome c reductases were determined to be distributed as in mammalian mitochondria. In contrast with the mammalian systems, fumarase and NAD-linked "malic" enzyme were isolated primarily from the intermembrane space fraction of the worm mitochondria. These enzymes are required for the anaerobic energy-generating system in Ascaris and would be expected to give rise to NADH in the intermembrane space. The need for and possible mechanism of a proton translocation system to obtain energy generation is suggested.