Searching for alternative sources of postnatal human mesenchymal stem cells: Candidate MSC-like cells from umbilical cord
Searching for alternative sources of postnatal human mesenchymal stem cells: Candidate MSC-like cells from umbilical cord
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DOI：
10.1634/stemcells.21-1-105
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发表时间：
2003-01-01
期刊：
影响因子：
5.2
通讯作者：
Smirnov, VN
中科院分区：
文献类型：
--
作者：
Romanov, YA;Svintsitskaya, VA;Smirnov, VN
间充质干细胞(MSCs)具有更新和分化为多种间充质组织谱系的能力。在几种人类疾病的细胞疗法的背景下，MSCs的这些特征引起了研究人员的极大关注。尽管骨髓是MSCs的主要来源，但由于病毒感染程度高，细胞数量和增殖/分化能力随着年龄的增长而显著下降，使用骨髓来源的细胞并不总是被接受。因此，对可能的替代MSC源的搜索仍有待验证。脐带血是造血干/祖细胞的丰富来源，不含间充质祖细胞。然而，MSCs在早产儿的血液中循环，并可能成功分离和扩增。这些细胞在妊娠末期的归宿尚不清楚。在这项研究中，我们尝试使用两种标准的方法从脐带静脉内皮下层分离MSCs：常规分离人脐静脉内皮细胞方案和在适合于骨髓来源的MSCs的条件下培养分离的细胞。我们的结果提示，脐带血管含有大量形成成纤维细胞集落的MSC样分子，这些集落可以在培养中成功扩增。这些间充质干细胞样细胞不含内皮或白细胞特异性抗原，但表达α-平滑肌肌动蛋白和几种间充质细胞标志。因此，脐带/胎盘基质可作为MSCs的替代来源，以满足实验和临床的需要。
Mesenchymal stem cells (MSCs) have the capability for renewal and differentiation into various lineages of mesenchymal tissues. These features of MSCs attract a lot of attention from investigators in the context of cell-based therapies of several human diseases. Despite the fact that bone marrow represents the main available source of MSCs, the use of bone marrow-derived cells is not always acceptable due to the high degree of viral infection and the significant drop in cell number and proliferative/differentiation capacity with age. Thus, the search for possible alternative MSC sources remains to be validated. Umbilical cord blood is a rich source of hematopoietic stem/progenitor cells and does not contain mesenchymal progenitors. However, MSCs circulate in the blood of preterm fetuses and may be successfully isolated and expanded. Where these cells home at the end of gestation is not clear. In this investigation, we have made an attempt to isolate MSCs from the subendothelial layer of umbilical cord vein using two standard methodological approaches: the routine isolation of human umbilical vein endothelial cell protocol and culture of isolated cells under conditions appropriate for bone-marrow-derived MSCs. Our results suggest that cord vasculature contains a high number of MSC-like elements forming colonies of fibroblastoid cells that may be successfully expanded in culture. These MSC-like cells contain no endothelium- or leukocyte-specific antigens but express alpha-smooth muscle actin and several mesenchymal cell markers. Therefore, umbilical cord/placenta stroma could be regarded as an alternative source of MSCs for experimental and clinical needs.