THE NUCLEOTIDE-SEQUENCE OF A HMW GLUTENIN SUBUNIT GENE LOCATED ON CHROMOSOME-1A OF WHEAT (TRITICUM-AESTIVUM L)
THE NUCLEOTIDE-SEQUENCE OF A HMW GLUTENIN SUBUNIT GENE LOCATED ON CHROMOSOME-1A OF WHEAT (TRITICUM-AESTIVUM L)
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DOI：
10.1093/nar/13.19.6817
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
MIFLIN, BJ
中科院分区：
文献类型：
--
作者：
FORDE, J;MALPICA, JM;MIFLIN, BJ
从普通小麦（Triticum aestivumL.）基因组文库中克隆了一个8.2kb的EcoRI片段。CV.夏延。该片段包含与谷蛋白的高分子量（HMW）亚基相关的序列，谷蛋白是被认为在决定面筋的弹性特性方面重要的蛋白质。克隆的HMW亚基基因似乎来自染色体1A。该基因的核苷酸序列为高分子量物质亚基的结构和进化提供了新的信息。然而，杂交选择翻译实验表明，这个基因是沉默的。
A cloned 8.2 kbEcoRI fragment has been isolated from a genomic library of DNA derived fromTriticum aestivumL. cv. Cheyenne. This fragment contains sequences related to the high molecular weight (HMW) subunits of glutenin, proteins considered to be important in determining the elastic properties of gluten. The cloned HMW subunit gene appears to be derived from chromosome 1A. The nucleotide sequence of this gene has provided new information on the structure and evolution of the HMW subunits. However, hybrid-selection translation experiments suggest that this gene is silent.