Cell-cell adhesion defects in Mrj mutant trophoblast cells are associated with failure to pattern the chorion during early placental development.
Cell-cell adhesion defects in Mrj mutant trophoblast cells are associated with failure to pattern the chorion during early placental development.
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Mrj 突变滋养层细胞的细胞间粘附缺陷与早期胎盘发育过程中绒毛膜模式失败有关。
DOI：
10.1002/dvdy.22755
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Cross,JamesC
中科院分区：
文献类型：
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作者：
Watson,EricaD;Hughes,Martha;Simmons,DavidG;Natale,DavidRC;Sutherland,AnnE;Cross,JamesC
Early placental development in mice involves patterning of the chorion into distinct layers, though little is understood regarding the interactions that regulate its organization. Here we demonstrate that keratin aggregates found inMrj−/−chorionic trophoblast cells are associated with abnormal cell morphology, collapse of the actin cytoskeleton, E‐cadherin and β‐catenin misexpression and extracellular matrix (ECM) disorganization. Accordingly,Mrj−/−trophoblast cells in vitro are nonadherent and display erratic migratory behavior. These cells also fail to differentiate into syncytiotrophoblast cells sinceRhox4bexpression, a marker of syncytiotrophoblast progenitors, was maintained andGcm1,Synb, andSynaexpression failed to increase. This differentiation defect was not solely attributable to E‐cadherin misexpression or ECM disorganization. However, platingMrj‐deficient cells on exogenous laminin‐511 normalized their cell behavior. Lastly, we show thatMrj−/−chorions at embryonic day 8.5 have expandedRhox4bexpression domains and do not form normal layers of gene expression suggesting that chorion patterning requires Mrj. Developmental Dynamics 240:2505–2519, 2011. © 2011 Wiley Periodicals, Inc.
