Megakaryocyte potentiating factor as a tumor marker of malignant pleural mesothelioma: Evaluation in comparison with mesothelin
Megakaryocyte potentiating factor as a tumor marker of malignant pleural mesothelioma: Evaluation in comparison with mesothelin
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DOI：
10.1016/j.lungcan.2008.02.012
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发表时间：
2008-10-01
期刊：
影响因子：
5.3
通讯作者：
Naka, Tetsuji
中科院分区：
文献类型：
--
作者：
Iwahori, Kota;Osaki, Tadashi;Naka, Tetsuji
目的：恶性胸膜间皮瘤（MPM）的诊断缺乏早期可靠的血液检查。巨核细胞增强因子（MPF）和间皮素变体（MSLN），间皮素基因家族的成员，已经被研究作为MPM的候选血清标志物。我们建立了一种新的酶联免疫吸附试验（ELISA）系统，以比较MPF和MSLN在MPM和对照组中的诊断效果。实验设计：用ELISA检测27例MPM患者和129例对照组（包括肺癌患者和无症状石棉暴露受试者）的MPF和MSLN。MPM患者血清MPF和MSLN水平与各对照组相比显著升高。计算受试者工作特征曲线下面积（AUC），以区分MPM和肺癌、健康石棉暴露受试者和健康成人。血清MPF的AUC为0.879，临界值= 19.1 ng/ml（灵敏度= 74.1%，特异性= 90.4%），血清MSLN的AUC为0.713，临界值= 93.5 ng/ml（灵敏度= 59.3%，特异性= 86.2%）。MPF和MSLN值的AUC之间的比较显示MPF显著上级优于MSLN（p=0.025）。MPF与MSLN在MPM中的相关性显著（Pearson相关系数= 0.77; p< 0.001）。结论：MPF对MPM的诊断价值优于MSLN，尽管两者对MPM的特异性几乎相同。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Purpose: An early and reliable blood test is one deficiency in diagnosis of malignant pleural mesothelioma (MPM). Megakaryocyte potentiating factor (MPF) and mesothelin variants (MSLN), members of the mesothetin gene family, have been studied as candidate serum markers for MPM. We developed a novel enzyme-linked immunosorbent assay (ELISA) system to compare the diagnostic efficacy of MPF and MSLN in MPM and control groups.Experimental design: MPF and MSLN were assayed with ELISA in 27 consecutive MPM patients and 129 controls including patients with lung cancer and asymptornatic asbestos-exposed subjects.Results: Statistically significant elevation of serum MPF and MSLN levels was noted in MPM patients in comparison with every control group. The area under the receiver operating characteristic curve (AUC) was calculated for differentiation of MPM and lung cancer, healthy asbestos-exposed subjects, and healthy adults. While the AUC for serum MPF was 0.879, cutoff = 19.1 ng/ml (sensitivity = 74.1%, specificity = 90.4%), the AUC for serum MSLN was 0.713, cut-off = 93.5 ng/ml (sensitivity = 59.3%, specificity = 86.2%). Comparison between AUC for MPF and MSLN values shows that MPF is significantly superior to MSLN (p=0.025). Finally, there was a significant correlation between MPF and MSLN values for MPM (Pearson's correlation coefficient = 0.77; p< 0.001).Conclusions: These findings suggest that diagnostic value of MPF for MPM was better than that of MSLN although both markers showed almost equal specificity for MPM. (C) 2008 Elsevier Ireland Ltd. All rights reserved.