miR-199a-3p and miR-214-3p improve the overall survival prediction of muscle-invasive bladder cancer patients after radical cystectomy.
miR-199a-3p and miR-214-3p improve the overall survival prediction of muscle-invasive bladder cancer patients after radical cystectomy.
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DOI：
10.1002/cam4.1161
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发表时间：
2017-10
期刊：
影响因子：
4
通讯作者：
Jung K
中科院分区：
文献类型：
--
作者：
Ecke TH;Stier K;Weickmann S;Zhao Z;Buckendahl L;Stephan C;Kilic E;Jung K
为了改善根治性膀胱切除术（RC）后肌层浸润性膀胱癌（MIBC）患者的进一步治疗管理和随访计划的临床决策，迫切需要更好的预后预测准确性。本研究的目的是评估差异表达的microRNA（miRNAs）的有效性，根据先前的研究作为预后标志物的总生存期（OS）后，RC模型结合临床病理学数据。通过RT-qPCR测量了来自156名MIBC患者的福尔马林固定、石蜡包埋组织样本中6种miRNA（miR-100 - 5 p、miR-130 b-3 p、miR-141 - 3 p、miR-199 a-3 p、miR-205 - 5 p和miR-214 - 3 p）的表达，这些患者在3个泌尿外科中心接受了RC。根据组织可用性使用2000年至2013年的样本，随访至2016年6月。患者队列被随机分为训练集（n = 100）和测试集（n = 56）。来自相邻正常组织的73个样本用作对照。进行Kaplan-Meier、单变量和多变量考克斯回归以及决策曲线分析，以评估临床病理变量和miRNA与OS的相关性。与非恶性组织样本相比，在MIBC样本中发现了增加的（miR-130 b-3 p和miR-141 - 3 p）和减少的（miR-100 - 5 p、miR-199 a-3 p和miR-214 - 3 p）miRNA表达（P < 0.0001）。miR-199 a-3 p和miR-214 - 3 p是考克斯回归模型中OS的独立标志物，具有显著的临床病理学变量年龄、肿瘤状态和淋巴结状态。在两组中，这两种miRNA改善了具有临床病理学变量的预测模型。通过决策曲线分析证实了预测效益。总之，将两种miRNA纳入基于临床数据的模型中用于RC后MIBC患者的结局预测可能是提高预后准确性的有价值的方法。
To improve the clinical decision‐making regarding further treatment management and follow‐up scheduling for patients with muscle‐invasive bladder cancer (MIBC) after radical cystectomy (RC), a better prediction accuracy of prognosis for these patients is urgently needed. The objective of this study was to evaluate the validity of differentially expressed microRNAs (miRNAs) based on a previous study as prognostic markers for overall survival (OS) after RC in models combined with clinicopathological data. The expression of six miRNAs (miR‐100‐5p, miR‐130b‐3p, miR‐141‐3p, miR‐199a‐3p, miR‐205‐5p, and miR‐214‐3p) was measured by RT‐qPCR in formalin‐fixed, paraffin‐embedded tissue samples from 156 MIBC patients who received RC in three urological centers. Samples from 2000 to 2013 were used according to their tissue availability, with follow‐up until June 2016. The patient cohort was randomly divided into a training (n = 100) and test set (n = 56). Seventy‐three samples from adjacent normal tissue were used as controls. Kaplan–Meier, univariate and multivariate Cox regression, and decision curve analyses were carried out to assess the association of clinicopathological variables and miRNAs to OS. Both increased (miR‐130b‐3p and miR‐141‐3p) and reduced (miR‐100‐5p, miR‐199a‐3p, and miR‐214‐3p) miRNA expressions were found in MIBC samples in comparison to nonmalignant tissue samples (P < 0.0001). miR‐199a‐3p and miR‐214‐3p were independent markers of OS in Cox regression models with the significant clinicopathological variables age, tumor status, and lymph node status. The prediction model with the clinicopathological variables was improved by these two miRNAs in both sets. The predictive benefit was confirmed by decision curve analysis. In conclusion, the inclusion of both miRNAs into models based on clinical data for the outcome prediction of MIBC patients after RC could be a valuable approach to improve prognostic accuracy.
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影响因子：
3.7
作者：
Li X;Chen J;Hu X;Huang Y;Li Z;Zhou L;Tian Z;Ma H;Wu Z;Chen M;Han Z;Peng Z;Zhao X;Liang C;Wang Y;Sun L;Chen J;Zhao J;Jiang B;Yang H;Gui Y;Cai Z;Zhang X
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DOI：
10.4137/bic.s33380
发表时间：
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期刊：
Biomarkers in cancer
影响因子：
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作者：
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Burke HB
影响因子：
4.8
作者：
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通讯作者：
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影响因子：
9
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影响因子：
6.4
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通讯作者：
Sempere, Lorenzo F.