ADAPTATION AND MAJOR CHROMOSOMAL CHANGES IN POPULATIONS OF SACCHAROMYCES-CEREVISIAE
ADAPTATION AND MAJOR CHROMOSOMAL CHANGES IN POPULATIONS OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1007/bf00351736
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发表时间：
1992-07-01
期刊：
影响因子：
2.5
通讯作者：
WILKE, CM
中科院分区：
文献类型：
--
作者：
ADAMS, J;PUSKASROZSA, S;WILKE, CM
13个独立的酿酒酵母种群(9个单倍体和4个二倍体)在连续培养约1000代后，生长受到pH为6的缓冲液中有机磷浓度的限制。从这些种群中分离的克隆分析表明，在终止时，种群中存在许多大规模的染色体长度变异和重排。16条酵母染色体中有9条参与了这种变化。这些变化很少可以用酸性磷酸酶结构位点的拷贝数增加来解释。一些关于观察到的染色体长度变异的性质和频率的考虑使我们得出结论，它们是选择性有利的。
Thirteen independent populations of Saccharomyces cerevisiae (nine haploid and four diploid) were maintained in continuous culture for up to approximately 1000 generations, with growth limited by the concentration of organic phosphates in medium buffered at pH 6. Analysis of clones isolated from these populations showed that a number (17) of large-scale chromosomal-length variants and rearrangements were present in the populations at their termination. Nine of the 16 yeast chromosomes were involved in such changes. Few of the changes could be explained by copy-number increases in the structural loci for acid phosphatase. Several considerations concerning the nature and frequency of the chromosome-length variants observed lead us to conclude that they are selectively advantageous.