The elusive role of heparan sulfate in Toxoplasma gondii infection.
The elusive role of heparan sulfate in Toxoplasma gondii infection.
复制标题
硫酸乙酰肝素在弓形虫感染中的难以捉摸的作用。
DOI：
10.1016/j.molbiopara.2004.11.007
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Esko,JeffreyD
中科院分区：
文献类型：
--
作者：
Bishop,JosephR;Esko,JeffreyD
本研究旨在确定硫酸乙酰肝素(HS)脱乙酰酶/N-磺基转移酶1(NDST1)的干扰素硫酸基化对弓形虫在小鼠体内传播的作用。具体地说，研究了在同一小鼠体内，NDST1缺陷器官的感染速度是否比野生型器官慢。对12只野生型和NDST1基因敲除小鼠进行了感染性试验。贡地。在首次检测到肝脏感染的第3天处死小鼠。以脾、心为对照器官。结果显示，试验组和对照组的中位感染率基本相同。肝脏感染不受NDST1灭活的影响，乳腺感染也不受影响。为了解释这一结果，从NDST-1灭活组织中纯化的肝细胞和乳腺上皮细胞被评估HSN-硫酸盐化的丧失和抵抗感染的能力。分析表明，在N-硫酸盐化过程中，肝细胞和乳腺上皮细胞均表现出约50%的下降，同时T也下降。淋病感染。结论：NDST1可促进体外感染，但不能促进体内感染。
This study aimed to determine ifN-sulfation of heparan sulfate (HS) byN-deacetylase/N-sulfotransferase 1 (NDST1) plays a role forToxoplasma gondiitissue dissemination in mice. Specifically, whether organs deficient in NDST1 were infected at slower rate than wild type organs within the same mouse was investigated. 12 wild-type and NDST1-knockout mice were tested for infectivity withT. gondii. Mice were sacrificed on day 3 when infection of the liver was first detected. The spleen and heart were used as control organs. The results showed that median infection rate was virtually identical between experimental and control groups. Infection of the liver was unaffected by inactivation of NDST1, as well as that of mammary gland. In order to explain this result, purified hepatocytes and mammary epithelial cells from NDST-1 inactivated tissues were assessed for loss of HSN-sulfation and ability to resist infection. Analysis showed that both hepatocytes and mammary epithelial cells exhibited about 50% reduction inN-sulfation as well as reducedT. gondiiinfection. It is concluded that NDST1 facilitates infectionin vitrobut notin vivo.
影响因子：
3.7
作者：
Cattamanchi A;Dowdy DW;Davis JL;Worodria W;Yoo S;Joloba M;Matovu J;Hopewell PC;Huang L
通讯作者：
Huang L
硫酸乙酰肝素 N-磺基转移酶缺陷的中国仓鼠卵巢细胞突变体中硫酸不足的硫酸乙酰肝素。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bame,KJ;Esko,JD
通讯作者：
Esko,JD
影响因子：
15.9
作者：
Esko, JD;Lindahl, U
通讯作者：
Lindahl, U