Genetic tracing of the gustatory and trigeminal neural pathways originating from T1R3-expressing taste receptor cells and solitary chemoreceptor cells
Genetic tracing of the gustatory and trigeminal neural pathways originating from T1R3-expressing taste receptor cells and solitary chemoreceptor cells
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DOI：
10.1016/j.mcn.2008.04.011
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发表时间：
2008-08-01
影响因子：
3.5
通讯作者：
Abe, Keiko
中科院分区：
文献类型：
--
作者：
Ohmoto, Makoto;Matsumoto, Ichiro;Abe, Keiko
我们在小鼠T1R3基因启动子/增强子的控制下，建立了表达跨神经元示踪剂小麦胚芽凝集素（WGA）的转基因小鼠系。在味蕾中，WGA基因在t1r3阳性的甜味/鲜味味觉受体细胞中忠实表达。WGA蛋白不是横向转移到味蕾中承载突触、酸反应的III型细胞，而是直接转移到膝状神经节和结节节/岩神经节的一部分神经元，并进一步转移到孤立束核的前中央区域。此外，WGA在鼻上皮的孤立化感受器细胞中表达，并沿三叉神经感觉通路转移到脑干神经元。孤立化感受器细胞内源性表达T1R3和苦味感受器T2Rs。这一结果显示了受体表达的异常特征。因此，t1r3-WGA转基因小鼠揭示了来自味觉受体细胞的甜味/鲜味味觉通路和来自孤立化学受体细胞的三叉神经通路。(C) 2008爱思唯尔公司版权所有。
We established transgenic mouse lines expressing a transneuronal tracer, wheat germ agglutinin (WGA), under the control of mouse T1R3 gene promoter/enhancer. In the taste buds, WGA transgene was faithfully expressed in T1R3-positive sweet/umami taste receptor cells. WGA protein was transferred not laterally to the synapse-bearing, sour-responsive type III cells in the taste buds but directly to a subset of neurons in the geniculate and nodose/petrosal ganglia, and further conveyed to a rostro-central region of the nucleus of solitary tract. In addition, WGA was expressed in solitary chemoreceptor cells in the nasal epithelium and transferred along the trigeminal sensory pathway to the brainstem neurons. The solitary chemoreceptor cells endogenously expressed T1R3 together with bitter taste receptors T2Rs. This result shows an exceptional signature of receptor expression. Thus, the t1r3-WGA transgenic mice revealed the sweet/umami gustatory pathways from taste receptor cells and the trigeminal neural pathway from solitary chemoreceptor cells. (C) 2008 Elsevier Inc. All rights reserved.