Probing behavior of the tea green leafhopper on different tea plant cultivars.
Probing behavior of the tea green leafhopper on different tea plant cultivars.
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DOI：
10.1016/s1872-2032(07)60083-3
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发表时间：
2007-10-01
影响因子：
--
通讯作者：
Han, Baoyu
中科院分区：
文献类型：
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作者：
Jin, Miao;Han, Baoyu
利用直流电穿透图像仪(DC-EPG)研究了绿叶蝉Empoasca vitis(Gothe)在9个茶树品种上的探测行为。测定了叶蝉针刺刺入韧皮部时产生的7种波形：A，刺孔途径形成；S，刺入韧皮部并停留在韧皮部时流涎；C，针刺到达韧皮部前主动摄食；E和F，韧皮部被动摄食；R，休眠昆虫，刺入叶组织，NP不刺入。通过聚类分析，将供试的9个茶树品种按每只昆虫的探针数、波形持续时间或不同茶树品种不同波形的每个探针数的持续时间划分为3类。波形S、E和F与叶蝉的主要取食活动相关，可为预测茶树对叶蝉的抗性水平提供有价值的信息。根据S、E和F波形的持续时间以及包括各种波形在内的每个探头的持续时间，对9个茶树品种对叶蝉的抗性水平进行了排序。抗性排序为：龙井昌业>；杭单>；郑和大白茶>；千梅601>；红芽佛朔>；中茶102>；中茶302>；龙井43>；安吉白茶，与茶园9个茶树品种上叶蝉种群密度(为害)所确定的抗性水平相对应。我们的研究表明，这种简便易行的DC-EPG技术有可能成为预测茶树品种对茶叶蝉抗性的可靠工具。
The probing behaviors of the tea green leafhopper, Empoasca vitis (Gothe), on 9 tea cultivars were studied using video-text Direct Current-Electrical Penetration Graph, i.e., DC-EPG. The following 7 types of waveforms produced by the leafhopper stylet probing were determined: A, stylet pathway formation; S, salivation when stylets pierce into and stay in phloems; C, active ingestion before stylets reach phloems; E and F, passive ingestion in phloems; R, the insect resting with its stylet inserted into the leaf tissue and NP without probing. The 9 tested tea cultivars were categorized into 3 groups by the cluster analysis according to the number of probes per insect, waveform durations, or duration per probe of various waveforms on different tea cultivars. Waveforms S, E and F correlated to the main feeding activity of the leafhopper and may provide valuable information on predicting the resis-tance level of the tea plants to the leafhopper. The resistance level of the 9 tea cultivars to the leafhopper was ranked based on the durations of waveforms S, E and F, as well as the duration per probe including various waveforms. The ranking order of the resis-tance was: Longjingchangye > Hangdan > Zhenghedabaicha > Qianmei 601 > Hongyafoshuo > Zhongcha 102 > Zhongcha 302 > Longjing 43 > Anjibaicha, which corresponded to the resistance level determined by the population density (infestation) of the leaf-hopper on the 9 tea cultivars in the tea fields. Our study suggests that this simple and convenient DC-EPG technique might have great potential as a reliable tool to predict the resistance of tea cultivars to the tea leafhopper.