Aging, exercise, and endothelial progenitor cell clonogenic and migratory capacity in men
Aging, exercise, and endothelial progenitor cell clonogenic and migratory capacity in men
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DOI：
10.1152/japplphysiol.01183.2006
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发表时间：
2007-03-01
影响因子：
3.3
通讯作者：
DeSouza, Christopher A.
中科院分区：
文献类型：
--
作者：
Hoetzer, Greta L.;Van Guilder, Gary P.;DeSouza, Christopher A.
循环内皮祖细胞（EPCs）的数量和功能损伤被认为有助于血管老化和相关的心血管风险增加。我们测试了以下假设：1）健康，久坐的成年男性的EPC克隆和迁移能力随着年龄的增长而逐渐下降; 2）定期有氧运动将改善以前久坐的中年和老年男性的EPC克隆和迁移能力。从46名健康久坐男性中采集外周血样本：10名年轻人（26 +/- 1岁），15名中年人（47 +/- 1岁）和21名老年人（63 +/- 1岁）。分离单核细胞并预接种2天，并将非贴壁细胞进一步培养7天以确定EPC集落形成单位。使用改良的Boyden室测定EPCs的迁移活性。10名久坐不动的中年和老年男性（59 +/- 3岁）在3个月有氧运动干预前后进行了研究。中年男性（12 +/- 3）和老年男性（8 +/- 2）的EPC集落形成单位数量与年轻男性（40 +/- 7）相比低75%（P < 0.01）。中年男性和老年男性之间的菌落数没有差异。与年轻人（813 +/- 114）和中年人（760 +/-114）相比，老年人（453 +/- 72）的EPC迁移（荧光单位）显著减少。运动干预增加了（P < 0.05）先前久坐的中年和老年男性的EPC集落形成单位（10 +/- 3至22 +/- 5）和迁移活动（683 +/- 96至1，022 +/- 123）。这些结果提供了进一步的证据，老化对EPC功能有不利影响。然而，有规律的有氧耐力运动是一种有效的生活方式干预策略，可以改善中年和老年健康男性的EPC克隆形成和迁移能力。
Numerical and functional impairment of circulating endothelial progenitor cells (EPCs) is thought to contribute to vascular aging and the associated increase in cardiovascular risk. We tested the following hypotheses: 1) EPC clonogenic and migratory capacity decrease progressively with age in healthy, sedentary adult men; and 2) regular aerobic exercise will improve EPC clonogenic and migratory capacity in previously sedentary middle-aged and older men. Peripheral blood samples were collected from 46 healthy sedentary men: 10 young (26 +/- 1 yr), 15 middle-aged (47 +/- 1 yr), and 21 older (63 +/- 1 yr). Mononuclear cells were isolated and preplated for 2 days, and nonadherent cells were further cultured for 7 days to determine EPC colony-forming units. Migratory activity of EPCs was determined using a modified Boyden chamber. Ten sedentary middle-aged and older men (59 +/- 3 yr) were studied before and after a 3-mo aerobic exercise intervention. The number of EPC colony-forming units was similar to 75% lower (P < 0.01) in middle-aged (12 +/- 3) and older (8 +/- 2) compared with young (40 +/- 7) men. There was no difference in colony count between middle-aged and older men. EPC migration ( fluorescent units) was significantly reduced in older (453 +/- 72) compared with young (813 +/- 114) and middle-aged (760 +/- 114) men. The exercise intervention increased (P < 0.05) both EPC colony-forming units (10 +/- 3 to 22 +/- 5) and migratory activity (683 +/- 96 to 1,022 +/- 123) in previously sedentary middle-aged and older men. These results provide further evidence that aging adversely affects EPC function. Regular aerobic-endurance exercise, however, is an effective lifestyle intervention strategy for improving EPC clonogenic and migratory capacity in middle-aged and older healthy men.