DETECTION OF SMOKING-RELATED COVALENT DNA ADDUCTS IN HUMAN-PLACENTA
DETECTION OF SMOKING-RELATED COVALENT DNA ADDUCTS IN HUMAN-PLACENTA
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DOI：
10.1126/science.3941892
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发表时间：
1986-01-03
期刊：
影响因子：
56.9
通讯作者：
RANDERATH, K
中科院分区：
文献类型：
--
作者：
EVERSON, RB;RANDERATH, E;RANDERATH, K
用最近发展起来的免疫学和~(32)P标记后分析法研究了人足月胎盘中是否存在共价DNA化学加成产物(加合物)。吸烟者胎盘标本的DNA加合物水平在用苯并[a]芘二氢二醇环氧化物(BPDE-I)修饰的DNA抗体检测时略有增加，但没有统计学意义上的显著增加。后标记试验检测到几个修饰的核苷酸，其中一个(加合物1)与怀孕期间母亲吸烟密切相关。在17名吸烟者中有16人的胎盘组织中存在这种加合物，但在14名不吸烟者中只有3人存在。在吸烟者中，一般情况下，加合物1的水平与问卷调查和吸烟暴露强度的生化指标只有微弱的相关性，这表明个人易感因素的调节。加合物似乎来自一种芳香族致癌物质，但它可能不是由烟草烟雾中研究最深入的几种多环芳烃或芳香胺产生的。这些数据表明，吸烟与体内人类DNA的共价损伤有关。
The presence of covalent DNA chemical addition products (adducts) in human term placentas was investigated by recently developed immunologic and 32P-postlabeling assays. DNA from placental specimens of smokers showed a small but not statistically significant increase in adduct levels when tested by antibodies to DNA modified with a benzo[a]pyrene dihydrodiol epoxide (BPDE-I), the ultimate carcinogenic derivative of benzo[a]pyrene. The postlabeling assay detected several modified nucleotides, one of which (adduct 1) strongly related to maternal smoking during pregnancy. This adduct was present in placental tissue from 16 of 17 smokers, but only 3 of 14 nonsmokers. Among smokers, levels of adduct 1 in general were only weakly related to questionnaire and biochemical measures of the intensity of smoking exposures, which suggests modulation by individual susceptibility factors. The adduct seemed to be derived from an aromatic carcinogen, but it may not result from several of the most intensely studied polycyclic aromatic hydrocarbons or aromatic amines in tobacco smoke. The data show the association of cigarette smoking with covalent damage to human DNA in vivo.