Isolated heart perfusion according to Langendorff - Still viable in the new millennium
Isolated heart perfusion according to Langendorff - Still viable in the new millennium
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DOI：
10.1016/j.vascn.2006.05.006
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发表时间：
2007-03-01
影响因子：
1.9
通讯作者：
Schulz, Richard
中科院分区：
文献类型：
--
作者：
Skrzypiec-Spring, Monika;Grotthus, Bartosz;Schulz, Richard
离体灌注哺乳动物心脏制备于1897年由Oscar Langendorff建立。该方法是在1866年由Elias Cyon在德国莱比锡的Carl Ludwig生理学研究所建立的离体灌注蛙心的基础上发展起来的。在19世纪末和世纪初使用这两种方法进行的观察导致了重要的发现，为我们理解心脏生理学奠定了基础。这包括温度、氧和钙离子对心脏收缩功能的作用，心房中心脏电活动的起源，迷走神经刺激的负性变时效应和乙酰胆碱在迷走神经中的冲动的化学传递。Langendorff自己证明了心脏通过冠状动脉从血液中获得营养和氧气，心脏的机械功能通过冠状动脉的变化来反映。该方法经历了许多修改，但其一般原理至今仍然相同。血液，或更常见的晶体灌注液，通过插入升主动脉中的carmula以恒定压力或恒定流量输送到心脏。主动脉中的逆行流动关闭主动脉瓣的小叶，因此，整个灌注液经由主动脉根部的口进入冠状动脉。在通过冠状动脉循环后，过硫酸盐通过冠状窦排入右心房.分离的哺乳动物心脏制备的简单性，可以使用这种方法进行的广泛的测量，其高重复性和相对低的成本使其成为现代心血管和药理学研究中非常有用的工具，尽管有一些缺点。在过去的十年中，该方法在许多领域取得了重要进展，包括缺血再灌注损伤，基于细胞的治疗和供心保存移植。(c)2006年爱思唯尔公司All rights reserved.
The isolated perfused mammalian heart preparation was established in 1897 by Oscar Langendorff The method was developed on the basis of the isolated perfused frog heart established by Elias Cyon at the Carl Ludwig Institute of Physiology in Leipzig, Germany in 1866. Observations made using both methods at the end of the 19th and at the beginning of the 20th century led to important discoveries, forming the basis for our understanding of heart physiology. This included the role of temperature, oxygen and calcium ions for heart contractile function, the origin of cardiac electrical activity in the atrium, the negative chronotropic effect of vagus stimulation and the chemical transmission of impulses in the vagus nerve by acetylcholine. Langendorff himself demonstrated that the heart receives its nutrients and oxygen from blood via the coronary arteries and that cardiac mechanical function is reflected by changes in the coronaryThe method underwent many modifications but its general principle remains the same today. Blood, or more commonly crystalloid perfusates, are delivered into the heart through a carmula inserted in the ascending aorta, either at constant pressure or constant flow. Retrograde flow in the aorta closes the leaflets of the aortic valve and as a consequence, the entire perfusate enters the coronary arteries via the ostia at the aortic root. After passing through the coronary circulation the perfasate drains into the right atrium via the coronary sinus.The simplicity of the isolated mammalian heart preparation, the broad spectrum of measurements which can be done using this method, its high reproducibility and relatively low cost make it a very useful tool in modem cardiovascular and pharmacological research, in spite of a few shortcomings. In the last decade the method has brought many important advances in many areas including ischemia-reperfusion injury, cell-based therapy and donor heart preservation for transplant. (c) 2006 Elsevier Inc. All rights reserved.