Vasoactive agents affect mesangial cell adhesion.
Vasoactive agents affect mesangial cell adhesion.
复制标题
血管活性剂影响系膜细胞粘附。
DOI：
10.1152/ajpcell.1986.251.4.c505
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Venkatachalam,MA
中科院分区：
文献类型：
--
作者：
Kreisberg,JI;Venkatachalam,MA
培养的系膜细胞中应力纤维的形成和维持与肌球蛋白轻链磷酸化有关[Kreisberg et al. 2017]。是。 J.生理学。 249（肾液电解质生理学。18）：F227-F235，1985]，肌动蛋白-肌球蛋白相互作用激活的生化指示剂。提高细胞内腺苷 3',5'-环单磷酸 (cAMP)（例如异丙肾上腺素）水平的药物会使应激纤维断裂并导致肌球蛋白轻链去磷酸化，而添加收缩剂如精氨酸加压素 (AVP) 和前列腺素 E2 (PGE2) 可逆转这些变化。由于培养细胞中应力纤维的发育与细胞与基质的紧密粘附相关，因此我们想检查血管活性剂是否对系膜细胞粘附有影响。通过胰蛋白酶测定测量，异丙肾上腺素和二丁酰 cAMP (DBcAMP) 均降低系膜细胞粘附（分离细胞百分比：对照 11 +/- 2.4%；异丙肾上腺素加异丁基甲基黄嘌呤 (IBMX) = 48.3 +/- 7.4%；DBcAMP = 29.3 +/- 3.7%；DBcAMP-IBMX = 73 +/- 4.4%）。通过干涉反射显微镜观察到的细胞和基质之间的焦点（粘附）接触区域也减少了，被更大分离的区域所取代（与基质接触的表面的百分比：对照= 7.4 +/- 0.8%；异丙肾上腺素-IBMX = 2.9 +/- 1.1%）。将 PGE2 或 AVP 添加到含有 cAMP 升高剂的培养介质中可防止上述变化。单独使用 PGE2 或 AVP 可增加系膜细胞粘附（脱落细胞百分比：对照 11 +/- 2.4%；PGE2 = 6.8 +/- 0.5%；AVP = 5.1 +/- 1.2%）。（摘要截断为 250 字）
The formation and maintenance of stress fibers in cultured mesangial cells is associated with myosin light chain phosphorylation [Kreisberg et al. Am. J. Physiol. 249 (Renal Fluid Electrolyte Physiol. 18): F227-F235, 1985], a biochemical indicator for activation of actin-myosin interactions. Agents that elevate intracellular levels of adenosine 3',5'-cyclic monophosphate (cAMP) (e.g., isoproterenol) fragment stress fibers and cause myosin light chain dephosphorylation, whereas the addition of contractile agents such as arginine vasopressin (AVP) and prostaglandin E2 (PGE2) reverses these changes. Because stress fiber development in cultured cells is correlated with tight cell to substrate adhesion, we wanted to examine whether vasoactive agents have an effect on mesangial cell adhesion. Both isoproterenol and dibutyryl cAMP (DBcAMP) reduced mesangial cell adherence as measured by a trypsin assay (% detached cells: control 11 +/- 2.4%; isoproterenol plus isobutylmethylxanthine (IBMX) = 48.3 +/- 7.4%; DBcAMP = 29.3 +/- 3.7%; DBcAMP-IBMX = 73 +/- 4.4%). The areas of focal (adhesive) contacts between the cell and substratum as observed by interference-reflexion microscopy were also reduced, being replaced by areas of greater separation (% of the surface in contact with the substratum: control = 7.4 +/- 0.8%; isoproterenol-IBMX = 2.9 +/- 1.1%). Addition of PGE2 or AVP to the incubation medium containing the cAMP-elevating agents prevented the above changes. PGE2 or AVP alone increased mesangial cell adhesion (% detached cells: control 11 +/- 2.4%; PGE2 = 6.8 +/- 0.5%; AVP = 5.1 +/- 1.2%).(ABSTRACT TRUNCATED AT 250 WORDS)