VIRUS-SPECIFIC ANTIBODY-PRODUCING CELLS IN BLOOD AND CEREBROSPINAL-FLUID IN ACUTE JAPANESE ENCEPHALITIS
VIRUS-SPECIFIC ANTIBODY-PRODUCING CELLS IN BLOOD AND CEREBROSPINAL-FLUID IN ACUTE JAPANESE ENCEPHALITIS
复制标题
DOI：
10.1002/jmv.1890170310
复制
发表时间：
1985-01-01
影响因子：
12.7
通讯作者：
LAORPONGSE, T
中科院分区：
文献类型：
--
作者：
BURKE, DS;NISALAK, A;LAORPONGSE, T
在泰国北方流行性乙型脑炎（乙脑）期间，对28例疑似乙脑患者的脑脊液（CSF）白细胞和血白细胞进行了乙脑病毒（JEV）抗体的体外自发合成试验。16例患者经病理证实为JEV感染。未受刺激的外周血单核细胞白细胞或未受刺激的未分级脑脊液白细胞培养三天的上清液进行了测试，为JEV IgM和IgG抗体与同种型特异性的“抗体捕获”放射免疫测定。来自所有16名JEV感染患者的血液来源的白细胞和来自4名JEV感染患者的CSF来源的白细胞合成JEV抗体。来自所有12名中枢神经系统感染患者的血液来源和CSF来源的白细胞均不能合成JEV抗体。在急性乙脑临床病程的早期，可在血液和脑脊液中检测到病毒特异性抗体产生细胞。
During an epidemic of Japanese encephalitis (JE) in northern Thailand, cerebrospinal fluid (CSF) leukocytes and blood leukocytes from 28 patients with suspected JE were tested spontaneous in vitro synthesis of antibodies of JE virus (JEV). Sixteen patients were sebsequently proven to be infected with JEV. Superntant fluids of three-day cultures of unstimulated peripheral blood mononclear leukocytes or unstimulated unfractionated CSF leukocytes were tested for JEV IgM and IgG antibodies with isotype-specific "antibody capture" radioimmunoassays. Blood derived leukocytes from all sixteen JEV-infected patients and CSF-derived leukocytes from four JEV-infected patients synthesized JEV antibodies. Blood-derived and CSF-derived leukocytes from all 12 patients with central nervous system infections caused by agents other than JEV uniformly failed to synthesize JEV antibodies. Virus-specific antibody-producing cells can be detected in the blood and CSF early in the clinical course of acute JE.