Immunohistochemical localization of neurotrophins and neurotrophin receptors in human and mouse salivary glands
Immunohistochemical localization of neurotrophins and neurotrophin receptors in human and mouse salivary glands
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DOI：
10.1016/s0940-9602(98)80016-2
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发表时间：
1998-04-01
影响因子：
2.2
通讯作者：
Vega, JA
中科院分区：
文献类型：
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作者：
De Vicente, JC;Garcia-Suarez, O;Vega, JA
本研究采用免疫组织化学方法分析人和小鼠唾液腺中低亲和力（p75）和高亲和力（TrkA、TrkB和TrkC）神经营养因子受体蛋白的发生。此外，还研究了神经营养因子的存在。使用针对Trk蛋白的多克隆抗体对14个人（4个腮腺、6个下颂腺和4个舌下腺）和5个小鼠唾液腺进行了研究。免疫染色的强度自动计算和评价在任意单位的灰度levels.In人体组织中没有免疫反应性（IR）的评估抗原中观察到的浆液性或粘液腺泡细胞，虽然TrkA IR被发现在腺泡的下颌下腺。闰管细胞呈p75IR（舌下）和TrkA IR（腮腺）。所有腺体的纹状管和排泄管均呈p75、TrkA和TrkC阳性反应，但未检测到TrkB阳性反应。在小鼠腺体中，导管细胞显示对p75（下颂下）和Trks A和C（腮腺和下颂下）的IR，但舌下腺不显示。在小鼠颌下腺中发现的唯一神经营养因子是NGF，提示神经营养因子可能参与控制唾液腺上皮细胞的生理功能。
This study was undertaken to analyze the occurrence of low- (p75) and high-affinity (TrkA, TrkB and TrkC) neurotrophin receptor proteins in human and mouse salivary glands using immunohistochemistry. Furthermore, the presence of neurotrophins was also investigated. The study was carried out on 14 human (4 parotid, 6 submandibular and 4 sublingual glands) and 5 mouse salivary glands, using polyclonal antibodies against Trk proteins. The intensity of immunostaining was calculated automatically and evaluated in arbitrary units of grey levels.In human tissues no immunoreactivity (IR) for the assessed antigens was observed in the serous or mucous acinar cells, although TrkA IR was found in the acini of the submandibular gland. The cells of the intercalated ducts showed p75 IR (sublingual) and TrkA IR (parotid gland). The striated and excretory ducts displayed p75 IR, TrkA IR and TrkC IR in all glands, but TrkB IR was never detected. No neurotrophins were detected.In the mouse glands the ductal cells display IR for p75 (submandibular) and Trks A and C (parotid and submandibular) but not the sublingual gland. Acinar cells of the submandibular gland also show p75 IR. The only neurotrophin found in the mouse salivary glands was NGF (submandibular gland).These results suggest that neurotrophins may be involved in controlling the physiology of epithelial salivary cells.