Suppression of the immune response potentiates tadpole tail regeneration during the refractory period
Suppression of the immune response potentiates tadpole tail regeneration during the refractory period
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DOI：
10.1242/dev.033985
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发表时间：
2009-07-15
期刊：
影响因子：
4.6
通讯作者：
Kubo, Takeo
中科院分区：
文献类型：
--
作者：
Fukazawa, Taro;Naora, Yuko;Kubo, Takeo
再生能力因动物种类和发育阶段而异，但决定这种差异的因素尚不清楚。尽管非洲爪蟾蝌蚪尾巴具有很高的再生能力，但在“不应期”，这种能力会暂时丧失。本研究表明，在“不应”期和“再生”期，断尾在伤口残肢中引起了不同的免疫反应：在不应期，一些免疫相关基因的表达延迟或延长，而在再生期，其他免疫相关基因没有明显或短暂的表达。此外，免疫抑制剂治疗或敲低PU导致的免疫细胞耗竭均可引起免疫抑制。1在不应期显著恢复再生能力。这些发现表明，免疫反应在决定爪蟾蝌蚪尾巴的再生能力方面起着至关重要的作用。
Regenerative ability varies depending on animal species and developmental stage, but the factors that determine this variability remain unclear. Although Xenopus laevis tadpole tails possess high regenerative ability, this is transiently lost during the 'refractory period'. Here, we show that tail amputation evokes different immune responses in wound tail stumps between the 'refractory' and 'regeneration' periods: there was delayed or prolonged expression of some immune-related genes in the refractory period, whereas there was no obvious or transient expression of other immune-related genes in the regeneration periods. In addition, immune suppression induced by either immunosuppressant treatment or immune cell depletion by knockdown of PU. 1 significantly restored regenerative ability during the refractory period. These findings indicate that immune responses have a crucial role in determining regenerative ability in Xenopus tadpole tails.