Oncogenic Human Papillomavirus involvement in epithelial ovarian carcinoma among women in Morocco
Oncogenic Human Papillomavirus involvement in epithelial ovarian carcinoma among women in Morocco
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DOI：
10.6026/97320630015055
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发表时间：
2019-01-01
期刊：
影响因子：
1.9
通讯作者：
Ennaji, Moulay Mustapha
中科院分区：
文献类型：
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作者：
Hammou, Rahma Ait;Benhessou, Mustapha;Ennaji, Moulay Mustapha
上皮性卵巢癌（EOC）虽然罕见，但却是全世界女性最致命的妇科癌症。尽管其患病率很高，但很少有研究对全球HPV感染的患病率和决定因素进行评估。本研究的目的是利用PCR在卡萨布兰卡妇女中调查摩洛哥EOC患者中HPV- dna的存在，并检查摩洛哥人群中某些HPV基因型的患病率。我们对70例上皮性卵巢癌患者的新鲜活检进行了HPV检测研究。采用MY09/11和GP5+/6+引物实现PCR。采用PCR对HPV 6、11、16、18、31和33型进行基因分型。数据采用SPSS软件进行统计分析。因此，平均年龄为48.9岁（21-76岁）。浆液性腺癌（75.71%）和ⅲ期占多数。HPV阳性8例（11.42%）。HPV基因分型结果显示两种基因型优势：HPV 16（87.5%）和HPV 31（12.5）。未发现合并感染。约75%的阳性病例为浆液性囊腺癌，62.5%以上为FIGO晚期（III或IV期）。我们的研究表明，高危HPV感染可能在摩洛哥EOC患者中起主要作用。
Epithelial Ovarian cancer (EOC) although rare is the most lethal gynecological cancer in women worldwide. Despite its high prevalence few studies have been performed to evaluate the prevalence and determinants of HPV infection worldwide. The aim of the present study was to investigate the presence of HPV-DNA in Moroccan patients with EOC using PCR among women in Casablanca, and to examine the prevalence of some HPV genotypes in Moroccan population. We performed a study of HPV detection on Fresh biopsies of 70 epithelial ovarian cancer patients. PCR was realized using the MY09/11 and GP5+/6+ primers. Genotyping of HPV was performed by PCR type-specific for HPV 6, 11, 16, 18, 31, and 33.Data was statistically analyzed using SPSS software. Hence, the mean age was 48.9 years (range, 21-76 years). Serous adeno carcinoma (75.71%) and stage III of the disease represent the majority of cases. eight patients were HPV positive (11.42%).Results of HPV genotyping revealed predominance of two genotypes: HPV 16 (87.5%) and HPV 31(12.5).No co-infection identified. Approximately 75% of positive cases had a serous cystadeno carcinoma and more than 62,5% had FIGO advanced stage (III or IV).Our study showed that high-risk HPV infection could play a major role among patients with EOC in Morocco.