A serum factor that recalcifies demineralized bone is conserved in bony fish and sharks but is not found in invertebrates.
A serum factor that recalcifies demineralized bone is conserved in bony fish and sharks but is not found in invertebrates.
复制标题
重新钙化脱矿骨的血清因子在硬骨鱼和鲨鱼中保守，但在无脊椎动物中未发现。
DOI：
10.1007/s00223-005-0205-6
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Price,PA
中科院分区：
文献类型：
--
作者：
Hamlin,NJ;Ong,KG;Price,PA
We investigated the evolutionary origin of a serum activity that induces calcification within a type I collagen matrix, an activity previously described in rat and bovine serum. Serum was obtained from vertebrates with calcified tissues (bony fish and shark), vertebrates without calcified tissues (lamprey and hagfish), and three invertebrates (marine worm, crab, and sea urchin). Serum from the bony fish and shark proved to contain a potent nucleator of collagen calcification; like the previously described calcifying activity in rat serum, the fish and shark activities are both able to recalcify a demineralized rat tibia when tested in Dulbecco’s modified Eagle medium containing as little as 1.5% of the respective serum and have an apparent molecular weight of 50–150 kDa. No calcifying activity could be detected in any of several experimental tests of invertebrate or hagfish serum. Weak calcifying activity could be detected in lamprey serum, but calcification was restricted to the growth plate of the decalcified tibia, with no detectable calcification in the type I collagen of the midshaft. These studies reveal a correlation between the evolutionary timing of the appearance of calcified tissues in vertebrates and the appearance of the serum activity that initiates calcification within collagen and, therefore, support the hypothesis that this serum activity may play a role in normal calcification of bone.
