Interactions of dissolved humic substances with oppositely charged fluorescent dyes for tracer techniques
Interactions of dissolved humic substances with oppositely charged fluorescent dyes for tracer techniques
复制标题
DOI：
10.1016/j.watres.2015.08.039
复制
发表时间：
2015-11-15
期刊：
影响因子：
12.8
通讯作者：
Satoh, Hisashi
中科院分区：
文献类型：
--
作者：
Hafuka, Akira;Ding, Qing;Satoh, Hisashi
为了研究带相反电荷的荧光染料和溶解的腐殖质之间的相互作用，用溶解的腐殖质对荧光素和罗丹明 6G 进行荧光猝灭。结合系数通过Stern-Volmer方程获得。通过添加腐殖酸，罗丹明 6G 的荧光很大程度上被猝灭，并获得了非线性 Stern-Volmer 图。这种强烈的猝灭可能是由阳离子罗丹明6G和腐殖酸之间的静电相互作用引起的，并通过疏水排斥力而增强。相比之下，溶解的腐殖质对荧光素的猝灭和相互作用作用相对较弱。 (C) 2015 Elsevier Ltd. 保留所有权利。
To investigate interactions between oppositely charged fluorescent dyes and dissolved humic substances, fluorescence quenching of fluorescein and rhodamine 6G with dissolved humic substances was performed. Binding coefficients were obtained by the Stern-Volmer equation. The fluorescence of rhodamine 6G was largely quenched by the addition of humic acid and a non-linear Stern-Volmer plot was obtained. This strong quenching may be caused by the electrostatic interaction between cationic rhodamine 6G and humic acid and strengthened by the hydrophobic repulsion. In contrast, the quenching and interactive effects of dissolved humic substances for fluorescein were relatively weak. (C) 2015 Elsevier Ltd. All rights reserved.