Cutting Edge: Mechanical Forces Acting on T Cells Immobilized via the TCR Complex Can Trigger TCR Signaling
Cutting Edge: Mechanical Forces Acting on T Cells Immobilized via the TCR Complex Can Trigger TCR Signaling
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DOI：
10.4049/jimmunol.0900775
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发表时间：
2010-06-01
影响因子：
4.4
通讯作者：
Roffler, Steve R.
中科院分区：
文献类型：
--
作者：
Li, Ya-Chen;Chen, Bing-Mae;Roffler, Steve R.
由MHC呈递的抗原肽与TCR的接合激活特异性T细胞以控制感染。最近的理论研究表明，作用于TCR的机械力对于受体触发可能是重要的。在这项研究中，我们直接测试了这样的假设，即作用于TCR的物理力可以通过显微操作与表达工程化TCR配体的人工APC结合的单个T细胞来启动T细胞中的信号传导。我们发现，通过TCR复合物而不是其他表面受体作用于与APC结合的T细胞的机械力可以以Src激酶依赖的方式启动T细胞中的信号传导。我们的数据表明，T细胞是机械敏感的，当耦合到APC的TCR，并表明TCR可以作为一个mechanosensor。我们的数据为TCR功能提供了新的见解。免疫学杂志，2010，184：5959-5963。
Engagement of the TCR by antigenic peptides presented by the MHC activates specific T cells to control infections. Recent theoretical considerations have suggested that mechanical forces acting on the TCR may be important for receptor triggering. In this study, we directly tested the hypothesis that physical forces acting on the TCR can initiate signaling in T cells by micromanipulation of individual T cells bound to artificial APCs expressing engineered TCR ligands. We find that mechanical forces acting on T cells bound to APCs via the TCR complex but not other surface receptors can initiate signaling in T cells in an Src kinase-dependent fashion. Our data indicate that T cells are mechanically sensitive when coupled to APCs by the TCR and indicates that the TCR may act as a mechanosensor. Our data provide new insight into TCR function. The Journal of Immunology, 2010, 184: 5959-5963.