Isolation and characterization of Sphingomonas sp GTIN11 capable of carbazole metabolism in petroleum
Isolation and characterization of Sphingomonas sp GTIN11 capable of carbazole metabolism in petroleum
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DOI：
10.1016/s0006-291x(02)02183-6
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发表时间：
2002-09-20
影响因子：
3.1
通讯作者：
Kayser, KJ
中科院分区：
文献类型：
--
作者：
Kilbane, JJ;Daram, A;Kayser, KJ
从人工煤气厂（MGP）土壤中分离出一种细菌培养物，该细菌培养物具有代谢含氮杂环咔唑的能力。该培养物被鉴定为鞘氨醇单胞菌属，并命名为GTIN 11。克隆的4.2 kb的DNA片段被证实含有负责咔唑降解的基因。DNA序列分析表明，该片段含有5个开放阅读框（ORF），推导的氨基酸序列与咔唑末端双加氧酶（ORF 1）、2，3-二羟基联苯双加氧酶亚基（ORF 2和ORF 3）、间位裂解复合物水解酶（ORF 4）和细菌多组分双加氧酶的铁氧蛋白组分（ORF 5）具有同源性。这些蛋白质与已知蛋白质的相似性百分比为61%或更低。测定了鞘氨醇单胞菌GTIN 11在37 ℃下降解咔唑的比活性为8.0 μ mol咔唑降解/min/g干细胞。该菌株在组成型表达咔唑降解特性方面是独特的。GTIN 11的休眠细胞去除95%的咔唑和50%的C1-咔唑从石油在16小时的处理时间。(C)2002年爱思唯尔科学（美国）。All rights reserved.
A bacterial culture was isolated from a manufactured gas plant (MGP) soil based on its ability to metabolize the nitrogen-containing heterocycle carbazole. The culture was identified as a Sphingonionas sp. and was given the designation GTIN11. A cloned 4.2 kb DNA fragment was confirmed to contain genes responsible for carbazole degradation. DNA sequence analysis revealed that the fragment contained five open reading frames (ORFs) with the deduced amino acid sequence showing homology to; carbazole terminal dioxygenase (ORFI), 2,3-dihydroxybiphenyl dioxygenase subunits (ORF2 and ORF3), meta-cleavage compound hydrolases (ORF4), and ferrodoxin component of bacterial multicomponent dioxygenases (ORF5). The percent similarity was 61% of these proteins or less to known proteins. The specific activity of Sphingomonas sp. GTIN11 for the degradation of carbazole at 37 degreesC was determined to be 8.0 mumol carbazole degraded/min/g dry cell. This strain is unique in expressing the carbazole degradation trait constitutively. Resting cells of Sphingomonas sp. GTIN11 removed 95% of carbazole and 50% of C1-carbazoles from petroleum in a 16-h treatment time. (C) 2002 Elsevier Science (USA). All rights reserved.