Anti-inflammatory activity of patchouli alcohol in RAW264.7 and HT-29 cells
Anti-inflammatory activity of patchouli alcohol in RAW264.7 and HT-29 cells
复制标题
DOI：
10.1016/j.fct.2012.12.062
复制
发表时间：
2013-05-01
影响因子：
4.3
通讯作者：
Lee, Seong-Ho
中科院分区：
文献类型：
--
作者：
Jeong, Jin Boo;Shin, Yong Kyu;Lee, Seong-Ho
广霍香醇(PA)是从薄荷科广霍香属植物广霍香属植物中提取的化合物。最近，有报道称PA抑制炎症介质的产生。然而，PA的抗炎作用的生物学机制尚未得到研究。在这项研究中，我们研究了PA是否通过下调NF-kappa B和ERK途径来减少炎症介质的产生。结果表明，PA可抑制脂多糖刺激的RAW264.7和肿瘤坏死因子-α刺激的HT-29细胞iNOS和IL-6在蛋白和mRNA水平的过表达。在脂多糖刺激的RAW264.7和肿瘤坏死因子-α刺激的HT-29细胞中，PA抑制I-kappa B-α的降解和p65核转位，进而抑制核因子-kappa B的转录活性。此外，PA还通过减少ERK1/2的磷酸化来抑制由内毒素或肿瘤坏死因子-α刺激的ERK1/2的激活。这些结果表明，PA通过抑制ERK介导的巨噬细胞和人结肠癌细胞中的NF-kappa B通路而具有抗炎作用。(C)2013爱思唯尔有限公司。保留所有权利。
Patchouli alcohol (PA) is a chemical compound extracted from patchouli which belongs to the genus Pogostemon, herb of mint family. Recently, it has been reported that PA inhibits the production of inflammatory mediators. However, the biological mechanisms of PA for anti-inflammatory activities have not been studied. In this study, we investigated whether PA decreases the production of inflammatory mediators through downregulation of the NF-kappa B and ERK pathway. Our data indicated that PA inhibits the overexpression of iNOS and IL-6 in protein and mRNA levels in LPS-stimulated RAW264.7 and TNF-alpha stimulated HT-29 cells. PA inhibited I kappa B-alpha degradation and p65 nuclear translocation, and subsequently suppressed transcriptional activity of NF-kappa B in LPS-stimulated RAW264.7 and TNF-alpha-stimulated HT-29 cells. In addition, PA inhibited LPS- or TNF-alpha-stimulated ERK1/2 activation by decreasing phosphorylation of ERK1/2. These findings suggest that PA shows anti-inflammatory activities through suppressing ERK-mediated NF-kappa B pathway in mouse macrophage and human colorectal cancer cells. (C) 2013 Elsevier Ltd. All rights reserved.