Effect of acemannan, an extracted polysaccharide from Aloe vera, on BMSCs proliferation, differentiation, extracellular matrix synthesis, mineralization, and bone formation in a tooth extraction model
Effect of acemannan, an extracted polysaccharide from Aloe vera, on BMSCs proliferation, differentiation, extracellular matrix synthesis, mineralization, and bone formation in a tooth extraction model
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DOI：
10.1007/s10266-012-0101-2
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发表时间：
2014-07-01
期刊：
影响因子：
2.5
通讯作者：
Thunyakitpisal, Pasutha
中科院分区：
文献类型：
--
作者：
Boonyagul, Sani;Banlunara, Wijit;Thunyakitpisal, Pasutha
芦荟是一种传统的伤口愈合药物。我们假设醋甘露聚糖，一种从芦荟凝胶中提取的多糖，可以影响骨形成。用不同浓度的乙酰甘露聚糖处理原代大鼠骨髓基质细胞（BMSCs）。新的DNA合成，VEGF，BMP-2，碱性磷酸酶活性，骨唾液蛋白，骨桥蛋白表达，矿化分别通过[H-3]胸苷掺入法，ELISA，生化分析，蛋白质印迹法，茜素红染色。在动物研究中，提取雄性Sprague-Dawley大鼠的下颌右切牙，并将醋甘露聚糖处理的海绵置于牙槽中。术后1周、2周、4周，解剖下颌骨。骨形成通过双能X线吸收测量法和组织病理学检查进行评价。体外实验结果显示，醋甘露聚糖显著增加BMSC增殖、VEGF、BMP-2、碱性磷酸酶活性、骨唾液蛋白和骨桥蛋白表达以及矿化。体内结果显示，与未治疗的对照组相比，乙酰甘露聚糖治疗组具有更高的骨矿物质密度和更快的骨愈合。在乙酰甘露聚糖治疗组中观察到大量骨小梁向内生长。这些数据表明醋甘露聚糖可以作为一种生物活性分子，通过刺激BMSCs增殖、分化成骨细胞和细胞外基质合成来诱导骨形成。醋甘露聚糖有望成为一种天然骨再生材料。
Aloe vera is a traditional wound healing medicine. We hypothesized acemannan, a polysaccharide extracted from Aloe vera gel, could affect bone formation. Primary rat bone marrow stromal cells (BMSCs) were treated with various concentrations of acemannan. New DNA synthesis, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein, osteopontin expression, and mineralization were determined by [H-3] thymidine incorporation assay, ELISA, biochemical assay, western blotting, and Alizarin Red staining, respectively. In an animal study, mandibular right incisors of male Sprague-Dawley rats were extracted and an acemannan treated sponge was placed in the socket. After 1, 2, and 4 weeks, the mandibles were dissected. Bone formation was evaluated by dual-energy X-ray absorptiometry and histopathological examination. The in vitro results revealed acemannan significantly increased BMSC proliferation, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein and osteopontin expression, and mineralization. In-vivo results showed acemannan-treated groups had higher bone mineral density and faster bone healing compared with untreated controls. A substantial ingrowth of bone trabeculae was observed in acemannan-treated groups. These data suggest acemannan could function as a bioactive molecule inducing bone formation by stimulating BMSCs proliferation, differentiation into osteoblasts, and extracellular matrix synthesis. Acemannan could be a candidate natural biomaterial for bone regeneration.