STUDIES ON LUTEAL GENERATION AND PROCESSING OF THE HIGH MOLECULAR WEIGHT RELAXIN PRECURSOR *
STUDIES ON LUTEAL GENERATION AND PROCESSING OF THE HIGH MOLECULAR WEIGHT RELAXIN PRECURSOR *
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高分子松弛素前体的黄体生成及加工研究*
DOI：
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发表时间：
1982
影响因子：
5.2
通讯作者：
M. Gast
中科院分区：
文献类型：
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作者：
M. Gast
生殖控制生物学是基础和临床科学家特别感兴趣的，因为受精和妊娠过程的药理学调节具有深远的影响。卵巢在微妙平衡的激素信号系统中占据中心位置，确保物种维持所必需的成功、重复的生殖结果。虽然卵巢产生类固醇已被广泛研究，但对卵巢分泌蛋白的机制知之甚少。由于松弛素是唯一被充分表征的卵巢分泌蛋白，我们对卵巢成熟和基因表达的兴趣促使我们使用松弛素合成作为研究这些卵巢细胞功能的工具。为此，我们在重组腹水无肿瘤细胞系统中利用卵巢RNAt的翻译试验。l 1978年，我们发现从妊娠母猪黄体中分离的RNA可以指导体外合成一个23000分子量（MW）的蛋白，其免疫学和序列与真正的松弛素相同。这种含有松弛素的蛋白（RCP）是卵巢松弛素生物合成的主要翻译产物。我们已经证明RCP是一个单链分子，包含松弛素的所有半胱氨酸肽，其结构与胰岛素前原分子相似。松弛素前体可能发生一系列的成熟修饰。这种修饰可能是合成、储存和释放过程的重要组成部分，负责将松弛素运送到目标组织。它们可能发生在几个亚细胞部位。我们选择检查前肽切割和膜易位，这是负责从RCP产生成熟松弛素的初始加工事件。由于松弛素是一种具有代表性的卵巢蛋白，我们对评估RCP信使RNA的细胞水平与RCP之间的关系感兴趣
The biology of reproductive control is of special interest to the basic and clinical scientist because of the far-reaching consequences of the pharmacologic regulation of the processes of fertilization and gestation. The ovary occupies a central position in the delicately balanced system of hormonal signals that insure the successful, repetitive reproductive outcomes necessary for species maintainence. Although steroid production by the ovary has been extensively studied, little is known of the mechanics of the ovarian elaboration of secretory proteins. Because relaxin is the only well-characterized ovarian secretory protein, our interest in ovarian maturation and gene expression led us to use relaxin synthesis as a tool to study these ovarian cellular functions. To do so, we utilized translational assay of ovarian RNAt in a reconstituted ascites tumor cell-free system.l In 1978 we showed that RNA isolated from corpora lutea of pregnant sows directs the synthesis in vitro of a 23,000 molecular weight (MW) protein with immunologic and sequence identity to authentic relaxin.*, This relaxincontaining-protein (or RCP) is the primary translation product of ovarian relaxin biosynthesis. We have since shown that RCP is a single chain molecule containing all of the cysteine-bearing peptides of relaxin and is structurally similar to the preproinsulin m01ecule.~ It is likely that a series of maturational modifications occur to the relaxin precursor. Such modifications may be an important part of the process of synthesis, storage, and release that are responsible for relaxin’s delivery to its target tissues. They probably occur at several subcellular sites. We chose to examine prepeptide cleavage and membrane translocation, the initial processing events responsible for the generation of mature relaxin from RCP. Because relaxin is a representative ovarian protein we were interested in evaluating the relationship between the cellular levels of the messenger RNA for RCP and
多巴胺能抑制培养的垂体细胞中催乳素合成和催乳素信使 RNA 积累。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Maurer,RA
通讯作者：
Maurer,RA
促肾上腺皮质激素/内啡肽及相关肽的结构和生物合成。
影响因子：
20.3
作者：
Eipper,BA;Mains,RE
通讯作者：
Mains,RE
从不同生殖状态的大鼠和成年循环猪中获得的卵巢提取物中的松弛素免疫活性水平。
影响因子：
4.8
作者：
Sherwood,OD;Rutherford,JE
通讯作者：
Rutherford,JE