SPECIES-SPECIFICITY AND ORGAN-SPECIFICITY OF SECRETORY PROTEINS DERIVED FROM HUMAN PROSTATE AND SEMINAL-VESICLES
SPECIES-SPECIFICITY AND ORGAN-SPECIFICITY OF SECRETORY PROTEINS DERIVED FROM HUMAN PROSTATE AND SEMINAL-VESICLES
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DOI：
10.1002/pros.2990170105
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发表时间：
1990-01-01
期刊：
影响因子：
2.8
通讯作者：
SCHEIT, KH
中科院分区：
文献类型：
--
作者：
AUMULLER, G;SEITZ, J;SCHEIT, KH
用抗人精囊液精胶蛋白(SG)和酸性磷酸酶(PAP)、β-微精蛋白(β-MSP)、人前列腺液前列腺特异性抗原(PSA)的多克隆抗体，以及抗β-MSP的单抗，对不同物种不同器官中的相应抗原进行免疫细胞化学检测。在青春期和成年人的精囊、输精管壶腹部和射精管的上皮细胞中检测到SG免疫反应阳性。PAP、β-MSP和PSA免疫反应在青春期和成人人前列腺以及头骨和尾部猴子的前列腺中被检测到。除射精管近端PSA免疫反应较弱外，其余抗血清均不与精囊、壶腹和射精管上皮细胞反应。在所研究的非灵长类动物中(狗、公牛、大鼠、豚鼠)，只有狗的前列腺上皮与PAP抗体呈明确的免疫反应，与PSA抗体呈中等反应。SG抗体在公牛、大鼠精囊和犬输精管壶腹部未见免疫反应。大鼠、公牛和狗的(腹侧)前列腺对β-MSP也是如此。大鼠前列腺背侧上皮与抗β-MSP的单抗和一种抗PSA的多克隆抗体有轻微的交叉反应。这些发现表明，人类精液蛋白的表达具有相当严格的物种依赖性，在灵长类动物中表现出一些相似之处，但与精液生理不同的物种之间只有微弱的亲缘关系。
Polyclonal antibodies against semenogelin (SG) isolated from human seminal vesicle secretion and acid phosphatase (PAP), .beta.-microseminoprotein (.beta.-MSP), and Prostate-Specific Antigen (PSA) derived from human prostatic fluid, as well as a monoclonal antibody against .beta.-MSP were used for immunocytochemical detection of the respective antigens in different organs from different species. SG-immunoreactivity was detected in the epithelium of the pubertal and adult human and in monkey seminal vesicle, ampulla of the vas deferens, and ejaculatory duct. PAP, .beta.-MSP, and PSA immunoreactivities were detected in the pubertal and adult human prostate and the cranial and caudal monkey prostate. With the exception of a weak PSA immunoreactivity in the proximal portions of the ejaculatory duct, none of the latter antisera reacted with seminal vesicle, ampullary, and ejaculatory duct epithelium. Among the non-primate species studied (dog, bull, rat, guinea pig) only the canine prostatic epithelium displayed a definite immunoreactivity with the PAP antibody and a moderate reaction with the PSA antibody. No immunoreaction was seen in bull and rat seminal vesicle and canine ampulla of the vas deferens with the SG antibody. The same was true for the (ventral) prostate of rat, bull, and dog for .beta.-MSP. The epithelium of the rat dorsal prostate showed a slight cross-reactivity with the monoclonal antibody against .beta.-MSP and one polyclonal antibody against PSA. The findings indicate a rather strict species-dependent expression of human seminal proteins which show some similarities in primates, but only marginal relationship to species with different physiology of seminal fluid.