Hepatocyte growth factor promotes proliferation and neuronal differentiation of neural stem cells from mouse embryos
Hepatocyte growth factor promotes proliferation and neuronal differentiation of neural stem cells from mouse embryos
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DOI：
10.1016/s1044-7431(03)00160-x
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发表时间：
2003-09-01
影响因子：
3.5
通讯作者：
Sakai, N
中科院分区：
文献类型：
--
作者：
Kokuzawa, J;Yoshimura, S;Sakai, N
肝细胞生长因子(HGF)最初是一种肝细胞有丝分裂原，最近被报道除了在神经系统的不同部位表达外，还具有神经营养活性。目前，HGF对神经干细胞(NSCs)的作用尚不清楚。在本研究中，我们首次报道了HGF对神经球的增殖和神经干细胞分化的促进作用。仅含HGF的培养基能诱导神经球的形成。在含有成纤维细胞生长因子2或表皮生长因子的培养液中加入HGF可以增加新形成的神经球的大小和数量。在分化培养液中加入HGF也可获得更多的神经元。相反，通过机械分离反复传代培养后，神经球的数量减少，这表明HGF形成的神经球主要由致力于神经元或神经胶质线的前体细胞组成。综上所述，这些结果表明，HGF促进神经球的增殖和小鼠胚胎来源的神经干细胞的神经元分化。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Hepatocyte growth factor (HGF), originally cloned as a hepatocyte mitogen, has recently been reported to exhibit neurotrophic activity in addition to being expressed in different parts of the nervous system. At present, the effects of HGF on neural stem cells (NSCs) are not known. In this study, we first report the promoting effect of HGF on the proliferation of neurospheres and neuronal differentiation of NSCs. Medium containing only HGF was capable of inducing neurosphere formation. Addition of HGF to medium containing fibroblast growth factor 2 or epidermal growth factor increased both the size and number of newly formed neurospheres. More neurons were also obtained when HGF was added in differentiation medium. In contrast, neurosphere numbers were reduced after repeated subculture by mechanical dissociation, suggesting that HGF-formed neurospheres comprised predominantly progenitor cells committed to neuronal or glial lines. Together, these results suggest that HGF promotes proliferation of neurospheres and neuronal differentiation of NSCs derived from mouse embyos. (C) 2003 Elsevier Science (USA). All rights reserved.