Bioactive hydrogel scaffolds for controllable vascular differentiation of human embryonic stem cells
Bioactive hydrogel scaffolds for controllable vascular differentiation of human embryonic stem cells
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DOI：
10.1016/j.biomaterials.2007.01.021
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发表时间：
2007-06-01
期刊：
影响因子：
14
通讯作者：
Langer, Robert
中科院分区：
文献类型：
--
作者：
Ferreira, Lino S.;Gerecht, Sharon;Langer, Robert
我们提出了一种新的方法来提高人胚胎干细胞（hESC）的血管分化的生物活性水凝胶封装。将hESC包封在含有或不含固定化调节因子的葡聚糖基水凝胶中：栓系的RGD肽和微囊化的VEGF（165）。表达血管内皮生长因子（VEGF）受体KDR/Flk-1，血管标记物的细胞的分数，增加了20倍，相比，自发分化的胚状体（EB）。与EB相比，具有表达外胚层标记物（包括巢蛋白）或内胚层标记物（包括甲胎蛋白）的调节因子的水凝胶中包封细胞的百分比分别降低2倍或3倍。当从这些网络中取出细胞并在有利于进一步血管分化的培养基条件下培养时，血管细胞的数量高于使用相同培养基条件通过EB获得的数量。因此，功能化的葡聚糖基水凝胶可以使大量的血管细胞，特别是内皮细胞的衍生，在组织工程和再生医学的潜在应用。(c)2007爱思唯尔有限公司保留所有权利。
We propose a new methodology to enhance the vascular differentiation of human embryonic stem cells (hESCs) by encapsulation in a bioactive hydrogel. hESCs were encapsulated in a dextran-based hydrogel with or without immobilized regulatory factors: a tethered RGD peptide and microencapsulated VEGF(165). The fraction of cells expressing vascular endothelial growth factor (VEGF) receptor KDR/Flk-1, a vascular marker, increased up to 20-fold, as compared to spontaneously differentiated embryoid bodies (EBs). The percentage of encapsulated cells in hydrogels with regulatory factors expressing ectodermal markers including nestin or endodermal markers including alpha-fetoprotein decreased 2- or 3-fold, respectively, as compared to EBs. When the cells were removed from these networks and cultured in media conditions conducive for further vascular differentiation, the number of vascular cells was higher than the number obtained through EBs, using the same media conditions. Functionalized dextran-based hydrogels could thus enable derivation of vascular cells in large quantities, particularly endothelial cells, for potential application in tissue engineering and regenerative medicine. (c) 2007 Elsevier Ltd. All rights reserved.