ATP-Triggered, Allosteric Self-Assembly of DNA Nanostructures
ATP-Triggered, Allosteric Self-Assembly of DNA Nanostructures
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DOI：
10.1021/jacs.9b10272
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发表时间：
2020-01-15
影响因子：
15
通讯作者：
Mao, Chengde
中科院分区：
文献类型：
--
作者：
Li, Qian;Liu, Longfei;Mao, Chengde
响应性自组装是生物系统中的一个普遍过程，在工程系统中是非常需要的。DNA纳米结构为研究这种反应性自组装提供了一个多功能的分子平台。人们已经探索了DNA纳米结构的各种触发因素。然而，每个触发器都需要一个独特的响应机制。这种情况给工程响应性带来了巨大的挑战。在此，我们提出了一种基于适配体的响应性DNA自组装的变构机制。适配体与配体的结合使DNA基序的构象发生改变，从而影响基序的组装。通过一个ATP适体模型，我们演示了响应组装。我们相信，这种反应性行为将成为分子机器、生物成像/生物传感和药物输送的重要元素。
Responsive self-assembly is a general process in biological systems and is highly desired in engineered systems. DNA nanostructures provide a versatile molecular platform for studying such responsive self-assembly. Various triggers have been explored for DNA nanostructures. However, each trigger requires a unique mechanism for its response. This situation brings a great challenge to engineer the responsiveness. Herein, we propose an aptamer-based, allosteric mechanism for responsive DNA self-assembly. The aptamer-ligand binding causes the DNA motif to change its conformation and thus influences the motif assembly. With a model of an ATP aptamer, we have demonstrated the responsive assembly. Such responsive behavior, we believe, will be an important element for molecular machines, bioimaging/biosensing, and drug delivery.