Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.
Sensitive in situ hybridisation technique using biotin-streptavidin-polyalkaline phosphatase complex.
复制标题
使用生物素-链霉亲和素-聚碱性磷酸酶复合物的灵敏原位杂交技术。
DOI：
10.1136/jcp.40.2.163
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发表时间：
1987
影响因子：
3.4
通讯作者：
C. Bird
中科院分区：
文献类型：
--
作者：
F. Lewis;S. Griffiths;R. Dunnicliff;M. Wells;N. Dudding;C. Bird
一个敏感的原位杂交技术，使用生物素-链霉亲和素-多聚碱性磷酸酶复合物检测系统，成功地应用于涂片新鲜培养的细胞，冷冻切片，福尔马林固定石蜡处理的组织：该程序是成功的DNA-DNA杂交使用各种DNA探针。该检测方法快速、可靠、经济，在杂交位点产生紫蓝色沉淀，并通过低倍光学显微镜清晰可见。
A sensitive in situ hybridisation technique, using a biotin-streptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA-DNA hybridizations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.