The dynein heavy chain gene family in Tetrahymena thermophila.
The dynein heavy chain gene family in Tetrahymena thermophila.
复制标题
嗜热四膜虫的动力蛋白重链基因家族。
DOI：
10.1111/j.1550-7408.1999.tb05136.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Pennock,DG
中科院分区：
文献类型：
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作者：
Xu,W;Royalty,MP;Zimmerman,JR;Angus,SP;Pennock,DG
动力蛋白ATPase是一个运动酶家族，驱动纤毛和鞭毛中的微管滑动，并有助于细胞内基于微管的运输。多动力蛋白假说提出了两个预测：1)轴丝含有多个动力蛋白重链(DHC)亚型，每个亚型由不同的基因编码；2)每个亚型在纤毛搏动中发挥特定的作用。我们利用基于聚合酶链式反应的技术从四膜体DNA中克隆了13个不同的DHC序列。所有13个基因似乎都在生长中的细胞中表达。与已知的13个DHs的氨基酸序列比较表明，我们已经克隆了3个外臂DHs。2个细胞质DHs和8个内臂DHs。
The dynein ATPases are a family of motor enzymes that drive microtubule sliding in cilia and flagella and contribute to microtubule‐based transport inside cells. the multi‐dynein hypothesis makes two predictions: 1) Axonemes contain multiple dynein heavy chain (DHC) isoforms, each encoded by a different gene; 2) Each isoform performs a specific role in ciliary beating. We used PCR‐based techniques to clone thirteen different DHC sequences fromTetrahymenagenomic DNA. All thirteen genes appeared to be expressed in growing cells. Comparisons of the deduced amino acid sequences of the thirteen DHCs with other known DHCs suggested that we have cloned three outer arm DHCs. two cytoplasmic DHCs, and eight inner arm DHCs.