Dual cellulose-digesting system of the wood-feeding termite, Coptotermes formosanus Shiraki
Dual cellulose-digesting system of the wood-feeding termite, Coptotermes formosanus Shiraki
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DOI：
10.1016/s0965-1748(01)00160-6
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发表时间：
2002-07-01
影响因子：
3.8
通讯作者：
Azuma, JI
中科院分区：
文献类型：
--
作者：
Nakashima, K;Watanabe, H;Azuma, JI
内-β-1,4-葡聚糖酶 (EG) 成分在食木白蚁 Coptotermes formosanus Shiraki 消化系统中的分布。使用聚丙烯酰胺凝胶电泳通过酶谱分析进行研究，然后进行 N 端蛋白质测序。与糖苷水解酶家族（GHF）9成员相似的EG成分仅限于唾液腺、前肠和中肠，而与GHF7成员相似的成分仅限于后肠，那里藏有大量纤维素分解鞭毛虫。 RT-PCR实验表明，5个与其他白蚁EG同源的GHF9 EG mRNA（1348 bp）在唾液腺和中肠中表达。从中肠和后肠制备的粗提取物从结晶纤维素产生葡萄糖。这些数据表明，C. formosanus 有两个独立的细胞纤维素消化系统：一个位于中肠，通过内源纤维素酶完成纤维素消化，另一个位于后肠，利用可能来自共生鞭毛虫的其他纤维素酶。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
The distribution of endo-beta-1,4-glucanase (EG) components in the digestive system of the wood-feeding termite, Coptotermes formosanus Shiraki. was investigated by zymogram analysis using polyacrylamide gel electrophoresis, followed by N-terminal protein sequencing. EG components similar to glycoside hydrolase family (GHF) 9 members were restricted to the salivary glands, the foregut, and the midgut, whereas components similar to GHF7 members were confined to the hindgut where numerous cellulolytic flagellates were harbored. RT-PCR experiments revealed that five GHF9 EG mRNAs (1348 bp) homologous to other termite EGs were expressed in the salivary glands and the midgut. The crude extract prepared from the midgut as well as that from the hindgut produced glucose from crystalline cellulose. These data suggest that C. formosanus has two independent cell ulose-digesting systems: one in the midgut where cellulose digestion is accomplished by endogenous cellulases and the other in the hindgut which makes use of other cellulases possibly from symbiotic flagellates. (C) 2002 Elsevier Science Ltd. All rights reserved.