Removal of nonspecifically bound proteins on microarrays using surface acoustic waves
Removal of nonspecifically bound proteins on microarrays using surface acoustic waves
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DOI：
10.1109/jsen.2008.917478
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发表时间：
2008-03-01
影响因子：
4.3
通讯作者：
Craighead, Harold G.
中科院分区：
文献类型：
--
作者：
Cular, Stefan;Branch, Darren W.;Craighead, Harold G.
蛋白质的非特异性结合是一个持续的问题，大大降低了生物传感器的灵敏度和选择性。我们证明，表面声波（SAW）装置产生的超声波可以从微阵列的传感和非传感区域去除非特异性结合的蛋白质。我们展示了我们的可控和非破坏性的清洁微阵列接口的方法。在这项工作中，使用128度YX锂酸盐产生SAW，选择它的高耦合系数和有效的功率转移到机械运动。这些波传播沿着的表面被耦合到特异性结合和非特异性结合的蛋白质在一个图案化的表面上的40 μ m的特征尺寸。使用荧光强度来量化微阵列的清洁功效。我们的研究结果表明，多余的蛋白质层和聚集体被去除，留下高度均匀的膜，如荧光强度曲线所证明的。选定的抗原-受体相互作用在声学清洁过程中保持结合，当经受11.25 mW的功率时，并保留其用于随后的抗原捕获的功效。结果表明，近完整的荧光信号恢复的传感和非传感区域的微阵列。重要的是，我们的方法可以集成到现有的阵列技术中，传感和非传感区域被广泛污染。我们相信，这项技术将在微传感器及其生物应用的发展和进步中发挥关键作用。
Nonspecific binding of proteins is an ongoing problem that dramatically reduces the sensitivity and selectivity of biosensors. We demonstrate that ultrasonic waves generated by surface acoustic wave (SAW) devices remove nonspecifically bound proteins from the sensing and nonsensing regions of the microarrays. We demonstrate our approach for controllably and nondestructively cleaning the microarray interface. In this work, SAWs were generated using 128 degrees YX lithium niobate, chosen for its high coupling coefficient and efficient power transfer to mechanical motion. These waves propagating along the surface were coupled into specifically bound and nonspecifically bound proteins on a patterned surface of 40 mu m feature size. Fluorescence intensity was used to quantify cleaning efficacy of the microarrays. Our results have shown that excess protein layers and aggregates are removed leaving highly uniform films as evidenced by fluorescence intensity profiles. Selected antigen-receptor interactions remained bound during the acoustic cleaning process when subjected to 11.25 mW of power and retained their efficacy for subsequent antigen capture. Results demonstrate near-complete fluorescence signal recovery for both the sensing and nonsensing regions of the microarrays. Of significance is that our approach can be integrated into existing array technologies where sensing and nonsensing regions are extensively fouled. We believe that this technology will be pivotal in the development and advancement of microsensors and their biological applications.