Fractures in cryopreserved elastic arteries
Fractures in cryopreserved elastic arteries
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DOI：
10.1006/cryo.1996.1997
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发表时间：
1997-03-01
期刊：
影响因子：
2.7
通讯作者：
Boylan, S
中科院分区：
文献类型：
--
作者：
Pegg, DE;Wusteman, MC;Boylan, S
本研究的目的是确定在低温保存过程中血管组织发生宏观断裂的条件，并开发一种实用的防止断裂的低温保存方法。对兔的颈总动脉进行冷冻保存，该冷冻保存过程已被优化以保留体外功能和细胞学结构。这涉及使用计算方案逐步添加和随后去除二甲基亚砜，该方案避免渗透损伤并最大限度地减少毒性作用、受控冷却、在-180摄氏度下储存和快速升温。其中75%的动脉严重断裂。系统地改变冷却和加热条件，以确定在冷却/储存/加热过程中何时发生断裂。然后使用差示扫描量热法来鉴定任何相应的热事件。结果发现，在该过程的升温阶段，当温度范围为-150至-100摄氏度时，发生断裂。发现所用冷冻保护剂的最大冷冻浓缩溶液的玻璃化转变温度为-123 ℃。降低储存温度（-180摄氏度）和-100摄氏度之间的升温速率，
The aim of this study was to define the conditions under which macroscopic fractures occur in vascular tissue during cryopreservation and to develop a practical cryopreservation method that prevents fracturing. The common carotid artery of the rabbit was subjected to a cryopreservation process that has been optimised for retention of in vitro function and cytological structure. This involves the stepwise addition and subsequent removal of dimethyl sulfoxide using a calculated protocol that avoids osmotic injury and minimises toxic action, controlled cooling, storage at -180 degrees C, and rapid warming. Seventy-five percent of such arteries were grossly fractured. The cooling and warming conditions were systematically varied to determine when in the cooling/storage/warming process the fractures occurred. Differential scanning calorimetry was then used to identify any corresponding thermal events. It was found that the fractures occurred as the temperature range -150 to -100 degrees C was traversed during the warming phase of the process. The glass transition temperature of a maximally freeze-concentrated solution of the cryoprotectant used was found to be -123 degrees C. Reducing the warming rate between the storage temperature (-180 degrees C) and -100 degrees C to