The P7-1 protein of southern rice black-streaked dwarf virus, a fijivirus, induces the formation of tubular structures in insect cells
The P7-1 protein of southern rice black-streaked dwarf virus, a fijivirus, induces the formation of tubular structures in insect cells
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南方水稻黑条矮缩病毒（一种“jivirus”）的 P7-1 蛋白诱导昆虫细胞中管状结构的形成
DOI：
10.1007/s00705-011-1041-9
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发表时间：
2011-10-01
影响因子：
2.7
通讯作者：
Wei, Taiyun
中科院分区：
文献类型：
--
作者：
Liu, Ying;Jia, Dongsheng;Wei, Taiyun
南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus，SRBSDV）是斐济病毒属（Fijivirus）的呼肠孤病毒属（reovirus），可引起植物和昆虫细胞中病毒管的形成。通过重组杆状病毒在昆虫细胞中表达SRBSDV的非结构蛋白P7-1，导致形成具有与在SRBSDV感染的细胞中发现的相似的尺寸和外观的小管。这些小管从细胞表面突出，支持P7-1蛋白含有两个推定的跨膜结构域的假设，这是必要的小管的形成。此外，SRBSDV P7-1蛋白的自身相互作用表明，该蛋白具有形成同源二聚体或寡聚体的能力，以组装所提出的螺旋对称结构的小管。综上所述，我们的结果表明，SRBSDV P7-1具有内在的自我相互作用的能力，在没有其他病毒蛋白的情况下形成从细胞表面生长的小管。
Southern rice black-streaked dwarf virus (SRBSDV), an insect- and plant-infecting reovirus of the genus Fijivirus, induced the formation of virus-containing tubules in infected plant and insect vector cells. Expression of the nonstructural protein P7-1 of SRBSDV in insect cells by a recombinant baculovirus resulted in the formation of tubules with dimensions and appearance similar to those found in SRBSDV-infected cells. These tubules protruded from the cell surface, supporting the hypothesis that the P7-1 protein contains two putative transmembrane domains that are necessary for the formation of tubules. Furthermore, the self-interaction of SRBSDV P7-1 protein indicates that this protein has the capacity to form homodimers or oligomers to assemble the proposed helical symmetry structure of tubules. Taken together, our results indicate that SRBSDV P7-1 has the intrinsic ability of self-interaction to form tubules growing from the cell surface in the absence of other viral proteins.